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Carp edema virus infection associated gill pathobiome: A case report

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F22%3AN0000089" target="_blank" >RIV/00027162:_____/22:N0000089 - isvavai.cz</a>

  • Alternative codes found

    RIV/62156489:43210/22:43921608 RIV/00216224:14310/22:00127769 RIV/62157124:16270/22:43880149

  • Result on the web

    <a href="https://onlinelibrary.wiley.com/doi/full/10.1111/jfd.13670" target="_blank" >https://onlinelibrary.wiley.com/doi/full/10.1111/jfd.13670</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1111/jfd.13670" target="_blank" >10.1111/jfd.13670</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Carp edema virus infection associated gill pathobiome: A case report

  • Original language description

    Understanding disease aetiology and pathologic mechanisms is essential for fish health evaluation. Carp edema virus (CEV) is the causative agent of a disease (CEVD) responsible for high mortality rates in both wild and cultured common carp Cyprinus carpio. Inspection of two carp specimens from a pond with high fish mortality revealed CEV infection in both the host and its ectoparasite (Argulus foliaceus). In addition to flavobacteria, well known to be associated with gill lesions, we found that free-living eukaryotes (amoebae and ciliates) and a temporary parasite (Ichthyobodo spp.) colonizing the gills may also contribute to alterations in gill structure and/or function, either directly, through firm (Ichthyobodo) or weak (amoebae) attachment of trophozoites to the gill epithelium, or indirectly, through carriage of pathogenic bacteria. Bacterial assemblages rich in families and genera, with predominance of Cetobacterium spp. in low-intensity alteration of the gill tissue and of Flavobacterium spp. in gills with extensive necrotic lesions, were detected in gills and within the cytoplasm of associated amoebae using high-throughput sequencing. Quantitative PCR indicated F. swingsii as the prevailing flavobacterial species within amoebae from less affected gills and F. psychrophilum within amoebae from extensively affected gills. This case study suggests that eukaryotic organisms as part of the gill pathobiome may also contribute to irreversible gill lesions seen in CEVD. Emphasizing the complexity of mutual relationships between bacterial assemblages and eukaryotic co-pathogens, further studies regarding factors that trigger pathology and influence severity in the CEV-positive carp are needed.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10606 - Microbiology

Result continuities

  • Project

    <a href="/en/project/EF16_019%2F0000869" target="_blank" >EF16_019/0000869: Sustainable production of healthy fish in various aquaculture systems - PROFISH</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2022

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Fish Diseases

  • ISSN

    0140-7775

  • e-ISSN

    1365-2761

  • Volume of the periodical

    45

  • Issue of the periodical within the volume

    10

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    9

  • Pages from-to

    1409-1417

  • UT code for WoS article

    000812282000001

  • EID of the result in the Scopus database

    2-s2.0-85131859826