First detection of Lake Sinai virus in the Czech Republic: a potential member of a new species

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F22%3AN0000113" target="_blank" >RIV/00027162:_____/22:N0000113 - isvavai.cz</a>

Alternative codes found

RIV/62157124:16270/22:43880470

Result on the web

<a href="https://link.springer.com/article/10.1007/s00705-022-05548-x" target="_blank" >https://link.springer.com/article/10.1007/s00705-022-05548-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00705-022-05548-x" target="_blank" >10.1007/s00705-022-05548-x</a>

Result language

angličtina

Original language name

First detection of Lake Sinai virus in the Czech Republic: a potential member of a new species

Original language description

Lake Sinai virus (LSV) is one of over 20 honey bee viruses. Variants of LSV have been classifed as members of two officially recognised species, Lake Sinai virus 1 and Lake Sinai virus 2. However, there are currently a limited number of whole-genome sequences, and the genetic variability of the virus indicates that additional species may need to be established. Extracted nucleic acid of 209 honey bee samples was screened by PCR for 11 honey bee viruses. LSV was the third most abundant virus (36.9% of positive samples), after Apis mellifera flamentous virus (72.2%) and deformed wing virus (52.5%). LSV-positive samples were analyzed further by PCR with primers targeting the region encoding the viral RNAdependent RNA polymerase. Subsequently, the PCR products were sequenced, and the resulting sequences were used for a frst round of phylogenetic analysis. Based on those results, several isolates were selected for whole-genome sequencing, and the complete genome sequences were used for additional phylogenetic analysis. The results indicated the presence of at least three genetically distinct groups of LSV in the Czech Republic, the most prevalent one being related to LSV 2 but too dissimilar to be considered a member of the same species. Two sequences of a major LSV strain cluster native to the Czech Republic were determined, representing the frst Czech LSV strains published to date.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10607 - Virology

Project

<a href="/en/project/QK1910286" target="_blank" >QK1910286: Effective procedures and strategies for managing of honey bee diseases and sustainable bee keeping</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Archives of Virology

ISSN

0304-8608

e-ISSN

1432-8798

Volume of the periodical

167

Issue of the periodical within the volume

11

Country of publishing house

AT - AUSTRIA

Number of pages

10

Pages from-to

2213–2222

UT code for WoS article

000836132200003

EID of the result in the Scopus database

2-s2.0-85135294340