Genetic diversity of Lake Sinai Virus in the Czech Republic

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F22%3A43880489" target="_blank" >RIV/62157124:16270/22:43880489 - isvavai.cz</a>
Result on the web
<a href="https://www.vfu.cz/files/upload/1401/KMVP%202022_Sbornik.pdf" target="_blank" >https://www.vfu.cz/files/upload/1401/KMVP%202022_Sbornik.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Genetic diversity of Lake Sinai Virus in the Czech Republic
Original language description
Lake Sinai virus (LSV) is a honey bee-infecting virus with two officially recognised species: LSV 1 and LSV 2. There is currently a limited number of whole-genome LSV sequences, yet the genetic variability of the virus indicates that there are more than two species in existence. Extracted nucleic acid of 209 honey bee samples were screened by PCR method for presence of LSV, which was found in 36.9% of the samples. LSV-positive samples underwent additional PCR reaction with primers targeting region coding RNA-dependent RNA polymerase of the virus. Subsequently, the PCR products were sequenced and the acquired sequences were used for first round of phylogenetic analysis. Several of the isolates were selected for whole-genome sequencing. Obtained sequences were used for additional phylogenetic analyses. The results indicate the presence of at least three genetically distinct groups of LSV in the Czech Republic. Finally, we obtained two LSV sequences via whole-genome sequencing.
Czech name
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Czech description
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Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
Sborník příspěvků XXIV. konference mladých vědeckých pracovníků s mezinárodní účastí
ISBN
978-80-7305-869-2
ISSN
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e-ISSN
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Number of pages
3
Pages from-to
68-70
Publisher name
Veterinární univerzita Brno
Place of publication
Brno
Event location
Brno
Event date
Jun 1, 2022
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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