Investigation on the colonisation of Campylobacter strains in the pig intestine depending on available metabolites
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F22%3AN0000143" target="_blank" >RIV/00027162:_____/22:N0000143 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0147957122001229?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0147957122001229?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.cimid.2022.101865" target="_blank" >10.1016/j.cimid.2022.101865</a>
Alternative languages
Result language
angličtina
Original language name
Investigation on the colonisation of Campylobacter strains in the pig intestine depending on available metabolites
Original language description
Campylobacter (C.) spp. represents one of the most important causes for food-borne bacterial pathogen in humans worldwide. The aim of this study was to investigate metabolic requirements of two Campylobacter strains of different species based on substrate utilisation (in vitro). Based on these results, a correlation between the colonisation and the available substrates in different intestinal sections was recorded using an animal model. Campylobacter coli (ST-5777) and C. jejuni (ST-122) were used to inoculate 16 pigs, respectively, and one group of 16 pigs was used as control. The strains differed significantly in substrate utilisation - C. coli was able to metabolise various substrates (acetate, asparagine, serine, fucose, and propionate), while C. jejuni only utilised serine. Metabolomic analysis of intestinal content from different gut sections showed the presence of all previously tested metabolites, except for fucose. A significantly larger amount of glucose was found in the jejunum of those pigs infected with C. coli, while neither strain utilised it in vitro. The analysis of the intestinal contents revealed a very low proportion of Campylobacterales in the total microbiome, suggesting that the small percentage of the inoculated Campylobacter strains in the gut microflora of the animals is too low to cause differences between the control and infected groups in the composition of the metabolome. Nevertheless, knowledge of specific nutritional requirements of the pathogens combined with proof of different metabolites in the intestinal segments may provide clues about the site of colonisation in the host and improve our understanding of this zoonotic germ.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40301 - Veterinary science
Result continuities
Project
<a href="/en/project/EF16_025%2F0007404" target="_blank" >EF16_025/0007404: Gut microbiota with probiotic potential for animal health and welfare</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Comparative Immunology, Microbiology and Infectious Diseases
ISSN
0147-9571
e-ISSN
1878-1667
Volume of the periodical
88
Issue of the periodical within the volume
September 2022
Country of publishing house
GB - UNITED KINGDOM
Number of pages
8
Pages from-to
"101865"
UT code for WoS article
000878499500001
EID of the result in the Scopus database
2-s2.0-85135123167