Interaction of bacteria-derived EVs with HEK293 cell clones
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F22%3AN0000179" target="_blank" >RIV/00027162:_____/22:N0000179 - isvavai.cz</a>
Result on the web
<a href="https://www.csaki.cz/odborne-akce/xxxix-sjezd-ceskych-a-slovenskych-alergologu-a-klinickych-imunologu-164" target="_blank" >https://www.csaki.cz/odborne-akce/xxxix-sjezd-ceskych-a-slovenskych-alergologu-a-klinickych-imunologu-164</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Interaction of bacteria-derived EVs with HEK293 cell clones
Original language description
Anti-pathogen innate immune responses, including TLRs, are triggered upon recognition of conserved pathogen-associated molecular patterns (PAMPs). TLR2 and TLR4 have been shown to play a role in sensing the surface structures of bacteria. Some of the PAMPs could be associated with the bacteria-derived extracellular vesicles (EVs) including lipoteichoic acid (LTA) and lipopolysaccharides (LPS). The aim of the study was the first insight into the interaction of Lacticaseibacillus rhamnosus- and Enterococcus faecium-derived EVs via TLRs using transfected HEK293 cells. Lacticaseibacillus rhamnosus-derived EVs consist of two populations of particles (30 40nm; 90-120nm) with a concentration of 1013 particles per ml. Enterococcus faecium-derived EVs size ranges between 100-150 nm in diameter with a concentration of 1013 particles per ml. Both bEVs were shown to contain LTA by western blotting. The functionality of transfected HEK clones was confirmed by flow cytometry and agonist receptor interaction. The cultivation of transfected HEK293 cells with Lacticaseibacillus rhamnosus-derived EVs Enterococcus faecium-derived EVs suggested the concentration-depended interaction with the TLR2/6 and TLR2/CD14 receptors. However, no interaction was detected with HEK293/TLR4-MD2-CD14 clone Lacticaseibacillus rhamnosus- and Enterococcus faecium-derived EVs seem to interact via TLR2/CD14 and TLR6/TLR2 receptors. On the other hand, no interaction was detected with HEK293/TLR4-MD2-CD14 clone.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
30107 - Medicinal chemistry
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů