Current efficacy of lysostaphin against Staphylococcus aureus strains relevant for burned patients

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F22%3AN0000260" target="_blank" >RIV/00027162:_____/22:N0000260 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Current efficacy of lysostaphin against Staphylococcus aureus strains relevant for burned patients
Original language description
Infectious complications play important role in morbidity and mortality in patients with burns. One of the most promising options, how we can manage the increasing resistance of pathogens, is the application of antibacterial enzymes called “enzybiotics”. The activity of lysostaphin was determined by the plate lysis assay and the turbidity assay. In the plate lysis assay, the bacterial suspension (108 CFU/mL) was plated on the blood agar, left to dry for 10 minutes and then lysostaphin was applied. In the turbidity assay, 10 µL of lysostaphin was added to 90 µL of the bacterial suspension (2 x 108 CFU/mL) in TSB in the microtiter plate. Then the turbidity of the culture medium was measured every 5 minutes during the 24-hour cultivation period to obtain bacterial growth curves. The plate lysis assay showed no bacterial growth even with the lowest concentration of lysostaphin tested (25 µg/mL) when freshly prepared lysostaphin was used. The antibacterial activity of lysostaphin stored for 12 weeks was lower, but still signiﬁcantly reduced bacterial growth.
Czech name
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Czech description
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Project
<a href="/en/project/NV19-05-00214" target="_blank" >NV19-05-00214: Study of therapeutic application of antibacterial wound dressing for skin and soft tissue infections in epidemiologically relevant strains of S. aureus - methicillin resistant.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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