Utilisation of Actiphage in combination with IS900 qPCR as a diagnostic tool for rapid determination of paratuberculosis infection status in small ruminant herds

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F23%3AN0000138" target="_blank" >RIV/00027162:_____/23:N0000138 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14310/23:00132730

Result on the web

<a href="https://sciendo.com/article/10.2478/jvetres-2023-0041" target="_blank" >https://sciendo.com/article/10.2478/jvetres-2023-0041</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2478/jvetres-2023-0041" target="_blank" >10.2478/jvetres-2023-0041</a>

Result language

angličtina

Original language name

Utilisation of Actiphage in combination with IS900 qPCR as a diagnostic tool for rapid determination of paratuberculosis infection status in small ruminant herds

Original language description

Introduction: Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of paratuberculosis, a chronic infectious intestinal disease occurring in domestic and wild ruminants. Early diagnosis of infected herds enabling timely adoption of control measures is tremendously important in view of the fact that the disease has a significant economic impact on farmers. The aim of this study was to evaluate the possibility of rapid detection of viable MAP on small ruminant farms based on environmental sample examination using a novel phage-based test named Actiphage. Material and Methods: A total of 9 fresh and 28 frozen (8 or 11 years at −70°C) environmental samples originating from paratuberculosis-affected farms were analysed for the presence of MAP by four different diagnostic methods: Actiphage combined with real-time PCR targeting insertion sequence 900 (IS900 qPCR), conventional phage amplification assay, culture (frozen samples only), and direct IS900 qPCR. Results: Viable MAP was detected in one fresh environmental sample using Actiphage–IS900 qPCR. None of the frozen samples tested positive using this diagnostic approach, which was consistent with the results of culture examination also providing information on viability. Conclusion: This study describes other possible and innovative uses of phage-based methods in paratuberculosis control strategies. The Actiphage-qPCR was found to be less laborious than culture and provided results within six hours, suggesting that it may be a valuable tool for rapid initial determination of the infectious status of farmed animals based on environmental sample examination.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

40301 - Veterinary science

Project

<a href="/en/project/QK1910082" target="_blank" >QK1910082: Addressing the problem of the occurrence of bacterial, protozoan and viral zoonotic agents in small ruminant breeds</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Veterinary Research

ISSN

2450-7393

e-ISSN

2450-8608

Volume of the periodical

67

Issue of the periodical within the volume

3

Country of publishing house

DE - GERMANY

Number of pages

6

Pages from-to

347-352

UT code for WoS article

001034204000001

EID of the result in the Scopus database

—