Limited value of current and new in silico predicted oocyst-specific proteins of Toxoplasma gondii for source-attributing serology

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F23%3AN0000219" target="_blank" >RIV/00027162:_____/23:N0000219 - isvavai.cz</a>

Alternative codes found

RIV/62157124:16170/23:43881092 RIV/62157124:16810/23:43881092

Result on the web

<a href="https://www.frontiersin.org/articles/10.3389/fpara.2023.1292322/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fpara.2023.1292322/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fpara.2023.1292322" target="_blank" >10.3389/fpara.2023.1292322</a>

Result language

angličtina

Original language name

Limited value of current and new in silico predicted oocyst-specific proteins of Toxoplasma gondii for source-attributing serology

Original language description

Toxoplasma gondii is a zoonotic parasite infecting all warm-blooded animals, including humans. The contribution of environmental contamination by T. gondii oocysts to infections is understudied. The aim of the current work was to explore T. gondii serology as a means of attributing the source of infection using a robust stepwise approach. We identified in silico thirty-two promising oocyst-specific antigens from T. gondii ´omics data, recombinantly expressed and purified them and validated whether serology based on these proteins could discriminate oocyst- from tissue cyst-driven experimental infections. For this, three well-characterized serum panels, sampled from 0 to 6 weeks post-infection, from pigs and sheep experimentally infected with T. gondii oocysts or tissue cysts, were used. Candidate proteins were initially screened by Western blot with sera from pigs or sheep, infected for different times, either with oocysts or tissue cysts, as well as non-infected animals. Only the recombinant proteins TgCCp5A and TgSR1 provoked seroconversion upon infection and appeared to discriminate between oocyst- and tissue cyst-driven infections with pig sera. They were subsequently used to develop an enzyme-linked immunosorbent assay test for pigs. Based on this assay and Western blot analyses, a lack of stage specificity and low antigenicity was observed with all pig sera. The same was true for proteins TgERP, TgSporoSAG, TgOWP1 and TgOWP8, previously described as source-attributing antigens, when analyzed using the whole panels of sera. We conclude that there is currently no antigen that allows the discrimination of T. gondii infections acquired from either oocysts or tissue cysts by serological tests. This work provides robust new knowledge that can inform further research and development toward source-attributing T. gondii serology.

Czech name

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Czech description

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Type

J_ost - Miscellaneous article in a specialist periodical

CEP classification

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OECD FORD branch

40301 - Veterinary science

Project

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Continuities

R - Projekt Ramcoveho programu EK

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Parasitology

ISSN

2813-2424

e-ISSN

2813-2424

Volume of the periodical

2

Issue of the periodical within the volume

2023

Country of publishing house

CH - SWITZERLAND

Number of pages

16

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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