Comparative Genomic Analysis and Characterization of Limosilactobacillus mucosae Strains Isolated from the Gastrointestinal Tract of Wild Boars in the Czech Republic

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F24%3AN0000061" target="_blank" >RIV/00027162:_____/24:N0000061 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Comparative Genomic Analysis and Characterization of Limosilactobacillus mucosae Strains Isolated from the Gastrointestinal Tract of Wild Boars in the Czech Republic

Original language description

Wild boars represent a promising source of suitable gut microbiota for use as probiotics in domestic pigs, particularly post-weaned piglets. The 15 strains of Limosilactobacillus mucosae obtained from the gastrointestinal tract (GIT) of wild boars were characterized in terms of their probiotic properties, while searching for coding sequences (CDS) of antibiotic resistance determinants and beneficial CDS, such as bacteriocins, adhesin-like factors, and carbohydrate-active enzymes. We also conducted a comparative genomic analysis of these 15 genomes of L. mucosae strains from our study and 49 genomes of L. mucosae obtained from the NCBI database. Average Nucleotide Identity analysis revealed that 9 out of the 15 isolates belonged to Limosilactobacillus sp., but not specifically to Limosilactobacillus mucosae sp., although 16S rRNA sequencing identified all 15 strains as L. mucosae. According to Abricate software, no antibiotic resistance genes were detected. When exploring beneficial properties of the strains, CDS encoding bacteriocin enterolysin A were found in 10 out of 15 strains. Regarding adhesin-like factors, CDS encoding lam29 and mub sequences were found in 15 and 12 strains, respectively. Additionally, CDS encoding type-4-prepilin peptidase were found in 7 strains, comGC in 4 strains, and fibronectin binding protein in 2 strains. Several important glycoside hydrolase families were identified, including GH13, GH36, GH43, and GH1. Notably, enzymes such as amylopullulanase (hydrolyzing starch), α-galactosidase, β-xylosidase, and α-arabinodase (degrading arabinoxylans), as well as 6-phospho-β-glucosidase (hydrolyzing cellobiose and gentiobiose), were found. The utilization of these carbohydrate substrates enhances feed intake and conversion. All strains of L. mucosae retrieved from the GIT of wild boars met safety requirements for probiotics by absence of resistance genes. The majority of the strains exhibited various beneficial probiotic properties, with CDS encoding enterolysin A and adhesin-like factors being prevalent. Notably, sequences lam29 and mub, typically associated with L. mucosae, were found in the majority of the strains. The utilization of various carbohydrate substrates was predicted through the discovery of glycoside hydrolases. This tested dataset holds promise for potential usage as probiotics for domestic pigs in husbandry.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

40301 - Veterinary science

Project

<a href="/en/project/QL24010234" target="_blank" >QL24010234: Innovative synbiotic feed supplement for postweaning piglets</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů