Genetic and phenotypic diversity of Flavobacterium psychrophilum isolates from Czech salmonid fish farms

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F24%3AN0000124" target="_blank" >RIV/00027162:_____/24:N0000124 - isvavai.cz</a>

Alternative codes found

RIV/62156489:43210/24:43925898 RIV/62157124:16170/24:43881381 RIV/62157124:16270/24:43881381 RIV/62157124:16810/24:43881381 and 2 more

Result on the web

<a href="https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-024-03490-3" target="_blank" >https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-024-03490-3</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12866-024-03490-3" target="_blank" >10.1186/s12866-024-03490-3</a>

Result language

angličtina

Original language name

Genetic and phenotypic diversity of Flavobacterium psychrophilum isolates from Czech salmonid fish farms

Original language description

Background: The salmonid pathogen Flavobacterium psychrophilum poses a significant economic threat to global aquaculture, yet our understanding of its genetic and phenotypic diversity remains incomplete across much of its geographic range. In this study, we characterise the genetic and phenotypic diversity of 70 isolates collected from rainbow trout (Oncorhynchus mykiss) and brown trout (Salmo trutta m. fario) from fish farms in the Czech Republic between 2012 and 2019 to compare their genomic content with all draft or complete genomes present in the NCBI database (n = 187). Results: The Czech isolates underwent comprehensive evaluation, including multiplex PCR-based serotyping, genetic analysis, antimicrobial resistance testing, and assessment of selected virulence factors. Multiplex PCR serotyping revealed 43 isolates as Type 1, 23 as Type 2, with sporadic cases of Types 3 and 4. Multi-locus sequence typing unveiled 12 sequence types (ST), including seven newly described ones. Notably, 24 isolates were identified as ST329, a novel sequence type, while 22 were classified as the globally-distributed ST2. Phylogenetic analysis demonstrated clonal distribution of ST329 in the Czech Republic, with these isolates lacking a phage sequence in their genomes. Antimicrobial susceptibility testing revealed a high proportion of isolates classified as non-wild type with reduced susceptibility to oxolinic acid, oxytetracycline, flumequine, and enrofloxacin, while most isolates were classified as wild type for florfenicol, sulfamethoxazole-trimethoprim, and erythromycin. However, 31 isolates classified as wild type for florfenicol exhibited minimum inhibitory concentrations at the susceptibility breakpoint. Conclusion: The prevalence of the Czech F. psychrophilum serotypes has evolved over time, likely influenced by the introduction of new isolates through international trade. Thus, it is crucial to monitor F. psychrophilum clones within and across countries using advanced methods such as MLST, serotyping, and genome sequencing. Given the open nature of the pan-genome, further sequencing of strains promises exciting discoveries in F. psychrophilum genomics.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

40103 - Fishery

Project

<a href="/en/project/EF16_019%2F0000869" target="_blank" >EF16_019/0000869: Sustainable production of healthy fish in various aquaculture systems - PROFISH</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

BMC Microbiology

ISSN

1471-2180

e-ISSN

1471-2180

Volume of the periodical

24

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

15

Pages from-to

"352"

UT code for WoS article

001315679100002

EID of the result in the Scopus database

2-s2.0-85204295092