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Comparative Genome Analysis and Characterization of the Probiotic Properties of Lactic Acid Bacteria Isolated from the Gastrointestinal Tract of Wild Boars in the Czech Republic

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F25%3AN0000071" target="_blank" >RIV/00027162:_____/25:N0000071 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216224:14310/25:00140264

  • Result on the web

    <a href="https://link.springer.com/article/10.1007/s12602-024-10259-7" target="_blank" >https://link.springer.com/article/10.1007/s12602-024-10259-7</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/s12602-024-10259-7" target="_blank" >10.1007/s12602-024-10259-7</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Comparative Genome Analysis and Characterization of the Probiotic Properties of Lactic Acid Bacteria Isolated from the Gastrointestinal Tract of Wild Boars in the Czech Republic

  • Original language description

    Probiotics are important components for maintaining a healthy gut microbiota in pigs, especially in post-weaned piglets. Lactic acid bacteria derived from the gastrointestinal tract of wild boars can serve as a source of beneficial probiotic strains with suitable properties for use in pig husbandry. In the study, we analyzed and characterized 15 strains of Limosilactobacillus mucosae obtained from the gut contents of wild boars to assess their safety and beneficial properties as probiotic candidates. The strains were compared using pan genomic analysis with 49 L. mucosae strains obtained from the NCBI database. All isolated strains demonstrated safety by absence of transferrable antimicrobial resistance genes and hemolysin activity. Based on the presence of beneficial genes, five candidates with probiotic properties were selected and subjected to phenotypic profiling. Five selected isolates exhibited the ability to survive conditions mimicking passage through the host’s digestive tract, such as low pH and the presence of bile salts. Furthermore, five selected strains demonstrated the presence of multiple carbohydrate-active enzymes and the ability to utilize various carbohydrate substrates. These strains can enhance the digestibility of oligo- or polysaccharide substrates found in food or feed, specifically resistant starch, α-galactosides, cellobiose, gentiobiose, and arabinoxylans. Based on the results obtained, the L. mucosae isolates tested in the study appear to be promising candidates for use as probiotics in pigs.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    40301 - Veterinary science

Result continuities

  • Project

    <a href="/en/project/QK1910351" target="_blank" >QK1910351: Wild boar and domestic pigs as a source of probiotic cultures and the influence of technological processes of preparation and synbiotic feed composition on its effectiveness and functionality</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2025

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Probiotics and Antimicrobial Proteins

  • ISSN

    1867-1306

  • e-ISSN

    1867-1314

  • Volume of the periodical

    17

  • Issue of the periodical within the volume

    4

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    19

  • Pages from-to

    1820-1838

  • UT code for WoS article

    001207122200002

  • EID of the result in the Scopus database

    2-s2.0-85191091250