The Effect of Testosterone, Nandrolone and Their Combination on the Structure and Ultrastructure of Muscle Fibres and Myofibrils in Pigs

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F25%3AN0000161" target="_blank" >RIV/00027162:_____/25:N0000161 - isvavai.cz</a>

Alternative codes found

RIV/62156489:43210/25:43926796

Result on the web

<a href="https://onlinelibrary.wiley.com/doi/10.1111/ahe.70033" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1111/ahe.70033</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

The Effect of Testosterone, Nandrolone and Their Combination on the Structure and Ultrastructure of Muscle Fibres and Myofibrils in Pigs

Original language description

There is increasing pressure on meat producers worldwide due to the need for higher yields and improved meat quality. This is why anabolic androgenic steroids (AAS) have been widely used in most countries, due to their ability to accelerate animal muscle growth. However, out of concern for their side effects, EU states have banned their use and implemented control mechanisms. But they are reaching their limits, and therefore, it is necessary to look for new ways and investigate the mechanism of action of AAS on muscle tissue. This study replicated the administration of banned AAS (testosterone, nandrolone and their combination) and observed their effect on pig muscle. The pig model was purposely chosen for the study, as no such research has been carried out on this species. At the same time, pork is one of the most consumed meats in Europe. It focused on histological changes in muscle structure, specifically the size of muscle fibres and the number of satellite cells per muscle fibre. Furthermore, ultrastructural changes in muscle fibres, the diameter of myofibrils, the number of myofibrils per area, the distance between myofibrils and the size of sarcomeres were examined. The results using the techniques of histology, fluorescent labelling and transmission electron microscopy showed that, after the application of AAS, there is an increase in the diameter of muscle fibres, an increase in the diameter of myofibrils, a decrease in the number of myofibrils per surface area and, in the case of testosterone, an increase in the distance between myofibrils and an increase in the length of sarcomeres. There was also a significant increase in the number of satellite cells per muscle fibre. The detected statistically significant differences between control and experimental groups provide evidence that selected histological parameters could be additional mechanisms for detecting the presence of AAS in pork meat in the future.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40301 - Veterinary science

Project

<a href="/en/project/QL24010272" target="_blank" >QL24010272: Omics as a modern tool for the detection of banned growth promoters in fattening food-producing animals</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Anatomia, Histologia, Embryologia

ISSN

0340-2096

e-ISSN

1439-0264

Volume of the periodical

54

Issue of the periodical within the volume

2

Country of publishing house

US - UNITED STATES

Number of pages

12

Pages from-to

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UT code for WoS article

001448683200001

EID of the result in the Scopus database

2-s2.0-105001034915