The role of extracellular vesicles in cell-to-cell communication

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F25%3AN0000201" target="_blank" >RIV/00027162:_____/25:N0000201 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

The role of extracellular vesicles in cell-to-cell communication

Original language description

Czech Annual Cancer Research Meeting 2025, ISBN 978-80-908348-4-2, Olomouc, 24.-26.11.2025, poster. Extracellular vesicles (EVs) are small particles surrounded by a lipid bilayer that cannot replicate on their own. They are released from cells into the external environment and are typically about 100 nm in size. Recent research shows that EVs are secreted from most cell types. In vitro cell cultures release EVs and other factors into the culture medium, creating what is known as conditioned cell culture medium. In the first part of our study, we compared the expression of specific cancer markers—such as EGFR, PD-L1, c-MET, and E-cadherin—between cell lines and the EVs derived from their conditioned medium. In the second part, we examined paired patient tissue samples (tumor and non-tumor) and the EVs isolated from them. EVs were extracted from the conditioned medium through ultracentrifugation, and the presence of these vesicles was confirmed using the CD63 marker. Subsequently, we measured the size, concentration and polydispersity index (PI) of the isolated EVs using multi-angle dynamic light scattering (MADLS). The characterization of EVs was further analyzed using flow cytometry analysis of small particles. Our results show that EGFR expression is consistent in samples from cell lines and EVs isolated from conditioned media. Both analyses indicate that EGFR levels are higher in tumor cell lines but lower in the non-tumor cell line, supporting the idea that EVs mirror the properties of the cells they come from. Elevated EGFR expression may suggest a tumor, but for accurate results, mutational analysis should be conducted to identify excessive or ongoing pathway stimulation. The expression levels of the CDH1 gene, which encodes E-cadherin, were analyzed in tissue patient samples using RT-qPCR. Notably, higher CDH1 expression was observed in tumor samples compared to non-tumor samples. Similarly, tissue EVs isolated from tumor samples showed higher E-cadherin expression compared to EVs from non-tumor samples. Our results showed that EVs isolated from conditioned media and tissue samples can contribute to intercellular communication, with the expression of examined proteins matching that of the originating cell sources.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

30203 - Respiratory systems

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů