Expression of Cancer Markers in Extracellular Vesicles: Comparison Between Cell Lines, Conditioned Media, and Patient Tissue Samples

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F25%3AN0000203" target="_blank" >RIV/00027162:_____/25:N0000203 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Expression of Cancer Markers in Extracellular Vesicles: Comparison Between Cell Lines, Conditioned Media, and Patient Tissue Samples

Original language description

CzeSEV meeting on Extracellular Vesicles 2025 (ISBN of Proceedings: 978-80-11-07777-8), Vestec, 11.11.2025, poster. Precise and dynamic cell–cell communication is fundamental for tissue homeostasis, development, immune surveillance, and disease progression. In addition to direct contact, cytokines and growth factors, along with extracellular vesicles (EVs), are also released from cells into the surrounding environment. In this study, we investigated whether EVs reflect the molecular profile of the original tumor cells. EVs were isolated by ultracentrifugation and characterized using MADLS and flow cytometry of small particles. Initially, we examined the expression of key oncogenic markers such as EGFR, PD-L1, c-MET, and E-cadherin in human cancer cell lines. EGFR expression levels were consistently elevated in both tumor cell lines and their corresponding EVs. These findings support the hypothesis that EVs faithfully recapitulate the protein expression patterns of their parental cells. Next, RT-qPCR analysis of EGFR, PD-L1, c-MET, and E-cadherin was performed in tissue samples from both non-tumor and tumor areas. Moreover, we compared the expression of these tumor markers on EVs isolated from the conditioned media of paired tumor and non-tumor tissue samples. Notably, E-cadherin, a marker associated with epithelial phenotype, was upregulated in tumor tissue samples, even in EVs. Our data demonstrate that EVs derived from both conditioned media and human tissue carry diagnostically relevant molecular information about their origin. These findings underscore the potential utility of EVs as minimally invasive biomarkers for tumor characterization and diagnostics.

Czech name

—

Czech description

—

Type

O - Miscellaneous

CEP classification

—

OECD FORD branch

30203 - Respiratory systems

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů