The choice of embedding media affects image quality, tissue R2*, and susceptibility behaviors in post-mortem brain MR microscopy at 7.0T
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064165%3A_____%2F19%3A10393283" target="_blank" >RIV/00064165:_____/19:10393283 - isvavai.cz</a>
Alternative codes found
RIV/00064190:_____/18:N0000096 RIV/00216208:11110/19:10393283 RIV/00064190:_____/19:N0000066
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=GDRe7_dS8O" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=GDRe7_dS8O</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/mrm.27595" target="_blank" >10.1002/mrm.27595</a>
Alternative languages
Result language
angličtina
Original language name
The choice of embedding media affects image quality, tissue R2*, and susceptibility behaviors in post-mortem brain MR microscopy at 7.0T
Original language description
Purpose: The quality and precision of post-mortem MRI microscopy may vary depending on the embedding medium used. To investigate this, our study evaluated the impact of 5 widely used media on: (1) image quality, (2) contrast of high spatial resolution gradient-echo (T-1 and T-2(*)-weighted) MR images, (3) effective transverse relaxation rate (R-2(*)), and (4) quantitative susceptibility measurements (QSM) of post-mortem brain specimens. Methods: Five formaldehyde-fixed brain slices were scanned using 7.0T MRI in: (1) formaldehyde solution (formalin), (2) phosphate-buffered saline (PBS), (3) deuterium oxide (D2O), (4) perfluoropolyether (Galden), and (5) agarose gel. SNR and contrast-to-noise ratii (SNR/CNR) were calculated for cortex/white matter (WM) and basal ganglia/WM regions. In addition, median R-2* and QSM values were extracted from caudate nucleus, putamen, globus pallidus, WM, and cortical regions. Results: PBS, Galden, and agarose returned higher SNR/CNR compared to formalin and D2O. Formalin fixation, and its use as embedding medium for scanning, increased tissue R-2*. Imaging with agarose, D2O, and Galden returned lower R-2* values than PBS (and formalin). No major QSM offsets were observed, although spatial variance was increased (with respect to R-2* behaviors) for formalin and agarose. Conclusions: Embedding media affect gradient-echo image quality, R-2*, and QSM in differing ways. In this study, PBS embedding was identified as the most stable experimental setup, although by a small margin. Agarose and Galden were preferred to formalin or D2O embedding. Formalin significantly increased R-2* causing noisier data and increased QSM variance.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30224 - Radiology, nuclear medicine and medical imaging
Result continuities
Project
<a href="/en/project/NV15-25602A" target="_blank" >NV15-25602A: Biomarkers of progression and treatment response in neurodegenerative disorders</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Magnetic Resonance in Medicine
ISSN
0740-3194
e-ISSN
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Volume of the periodical
81
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
14
Pages from-to
2688-2701
UT code for WoS article
000462092100039
EID of the result in the Scopus database
2-s2.0-85057769439