The choice of embedding media affects image quality, tissue R2 * , and susceptibility behaviors in post-mortem brain MR microscopy at 7.0T

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064190%3A_____%2F18%3AN0000096" target="_blank" >RIV/00064190:_____/18:N0000096 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/19:10393283 RIV/00064190:_____/19:N0000066 RIV/00064165:_____/19:10393283

Result on the web

<a href="https://doi.org/10.1002/mrm.27595" target="_blank" >https://doi.org/10.1002/mrm.27595</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/mrm.27595" target="_blank" >10.1002/mrm.27595</a>

Result language

angličtina

Original language name

The choice of embedding media affects image quality, tissue R2 * , and susceptibility behaviors in post-mortem brain MR microscopy at 7.0T

Original language description

PURPOSE: The quality and precision of post-mortem MRI microscopy may vary depending on the embedding medium used. To investigate this, our study evaluated the impact of 5 widely used media on: (1) image quality, (2) contrast of high spatial resolution gradient-echo (T1 and T2 * -weighted) MR images, (3) effective transverse relaxation rate (R2 * ), and (4) quantitative susceptibility measurements (QSM) of post-mortem brain specimens. METHODS: Five formaldehyde-fixed brain slices were scanned using 7.0T MRI in: (1) formaldehyde solution (formalin), (2) phosphate-buffered saline (PBS), (3) deuterium oxide (D2 O), (4) perfluoropolyether (Galden), and (5) agarose gel. SNR and contrast-to-noise ratii (SNR/CNR) were calculated for cortex/white matter (WM) and basal ganglia/WM regions. In addition, median R2 * and QSM values were extracted from caudate nucleus, putamen, globus pallidus, WM, and cortical regions. RESULTS: PBS, Galden, and agarose returned higher SNR/CNR compared to formalin and D2 O. Formalin fixation, and its use as embedding medium for scanning, increased tissue R2 * . Imaging with agarose, D2 O, and Galden returned lower R2 * values than PBS (and formalin). No major QSM offsets were observed, although spatial variance was increased (with respect to R2 * behaviors) for formalin and agarose. CONCLUSIONS: Embedding media affect gradient-echo image quality, R2 * , and QSM in differing ways. In this study, PBS embedding was identified as the most stable experimental setup, although by a small margin. Agarose and Galden were preferred to formalin or D2 O embedding. Formalin significantly increased R2 * causing noisier data and increased QSM variance.

Czech name

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Czech description

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Type

J_ost - Miscellaneous article in a specialist periodical

CEP classification

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OECD FORD branch

30103 - Neurosciences (including psychophysiology)

Project

<a href="/en/project/NV15-25602A" target="_blank" >NV15-25602A: Biomarkers of progression and treatment response in neurodegenerative disorders</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Magnetic Resonance in Medicine

ISSN

0740-3194

e-ISSN

1522-2594

Volume of the periodical

81

Issue of the periodical within the volume

4

Country of publishing house

US - UNITED STATES

Number of pages

14

Pages from-to

2688-2701

UT code for WoS article

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EID of the result in the Scopus database

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