Determination of retinoic acid in human serum and plasma by high-performance liquid chromatography

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064165%3A_____%2F19%3A10409783" target="_blank" >RIV/00064165:_____/19:10409783 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/19:10409783

Result on the web

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=LvFF_nJ--Y" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=LvFF_nJ--Y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00706-019-02489-1" target="_blank" >10.1007/s00706-019-02489-1</a>

Result language

angličtina

Original language name

Determination of retinoic acid in human serum and plasma by high-performance liquid chromatography

Original language description

All-trans retinoic acid (ATRA) is the main derivative of retinol. It is formed in the intestinal mucosa from retinaldehyde. The aim was to establish a high-performance liquid chromatography method for determination of ATRA in human serum and plasma. Stability of ATRA in serum stored under various conditions was tested as well. ATRA was extracted from serum (plasma) after deproteination with acetonitrile by hexan:ethylacetate mixture (50:50). The extract was then evaporated under nitrogen at room temperature and dissolved in methanol. ATRA was detected after separation on C18 column at 355 nm. This method uses acitretin as internal standard. Limit of detection was 0.21 µg/dm3 and limit of quantification 0.70 µg/dm3. The method is linear for ATRA concentration 0.78-25 µg/dm3. Based on our results, ATRA has the same concentration in serum and in plasma. Repeatability of the method measured within run is given by variation coefficient 4.31% for serum and 1.86% for plasma. Reproducibility of the method measured between runs is given by variation coefficient 9.55% for serum and 8.00% for plasma. The worst storage conditions were light and room temperature, ATRA decreased by 0.69 µg/dm3, i.e., by 39%. Simultaneously, with ATRA, retinol is measured as well. We have introduced a fast, simple, and reliable method for determination of ATRA in human serum and plasma.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Monatshefte für Chemie - Chemical Monthly

ISSN

0026-9247

e-ISSN

—

Volume of the periodical

150

Issue of the periodical within the volume

10

Country of publishing house

AT - AUSTRIA

Number of pages

5

Pages from-to

1731-1735

UT code for WoS article

000514591400001

EID of the result in the Scopus database

2-s2.0-85074212172