Determination of retinol and alpha-tocopherol in human seminal plasma using an HPLC with UV detection

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F14%3A39898252" target="_blank" >RIV/00216275:25310/14:39898252 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1111/and.12103" target="_blank" >http://dx.doi.org/10.1111/and.12103</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/and.12103" target="_blank" >10.1111/and.12103</a>

Result language

angličtina

Original language name

Determination of retinol and alpha-tocopherol in human seminal plasma using an HPLC with UV detection

Original language description

Oxidative stress has been proposed as one of the potential causes for infertility in men. Retinol and alpha-tocopherol have an important role in the spermatozoa defences against oxidative stress. A method is described here for the simultaneous determination of retinol and alpha-tocopherol in human seminal plasma with a suitable sample preparation procedure to prevent retinol and alpha-tocopherol degradation. After adequate sample preparation, the samples were determined by reversed-phase column chromatography with UV detection. The analytical performance of this method was satisfactory. The intra-assay and inter-assay coefficients of variation were below 10%. The recoveries were as follows: 90.7% (CV 8.1%) for retinol and 98.2% (CV 4.8%) for alpha-tocopherol. No significant differences in both retinol and alpha-tocopherol concentration between the smokers and nonsmokers (15 +/- 7nm and 1.86 +/- 0.29 mu m versus 15 +/- 6nm and 1.93 +/- 0.45 mu m) were found. A selective high-performance

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

—

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Andrologia

ISSN

0303-4569

e-ISSN

—

Volume of the periodical

46

Issue of the periodical within the volume

5

Country of publishing house

DE - GERMANY

Number of pages

7

Pages from-to

472-478

UT code for WoS article

000335009600003

EID of the result in the Scopus database

—