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Rapid determination of lipophilic vitamins in human serum by ultra-high performance liquid chromatography using a fluorinated column and high-throughput miniaturized liquid-liquid extraction

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F17%3A10365304" target="_blank" >RIV/00216208:11160/17:10365304 - isvavai.cz</a>

  • Alternative codes found

    RIV/00179906:_____/17:10365304

  • Result on the web

    <a href="http://onlinelibrary.wiley.com/doi/10.1002/jssc.201700492" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/jssc.201700492</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1002/jssc.201700492" target="_blank" >10.1002/jssc.201700492</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Rapid determination of lipophilic vitamins in human serum by ultra-high performance liquid chromatography using a fluorinated column and high-throughput miniaturized liquid-liquid extraction

  • Original language description

    A high-throughput miniaturized liquid-liquid extraction procedure followed by a simple ultra-high performance liquid chromatography method coupled with fluorescence detection for bioanalytical analysis of all tocopherol isomers and retinol in human serumhas been developed and validated. In the extraction procedure, a synthetic internal standard tocol was used, which does not occur in the human body. The separation of structurally related vitamins was achieved using a new generation of pentafluorophenyl propyl core-shell stationary phase with elution using methanol and an aqueous solution of ammonium acetate. The fluorescence of retinol and tocopherol isomers was detected at lambda(ex) = 325, 295 nm and lambda(em) = 480, 325 nm, respectively. The rapid baseline separation of all analytes was accomplished within 4.0 min. The sensitivity of method was demonstrated with lower limits of quantification: retinol 0.01 mu M, alpha-tocopherol 0.38 mu M, beta-tocopherol 0.18 mu M, gamma-tocopherol 0.14 mu M, and delta-tocopherol 0.01 mu M. Possible application of this method in clinical practice was confirmed by the analysis of human serum samples from healthy volunteers. Finally, the simultaneous determination of retinol and all tocopherol isomers in human serum can enable the clarification of their role in metabolism and in diseases such as cancer.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    30104 - Pharmacology and pharmacy

Result continuities

  • Project

  • Continuities

    S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2017

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Separation Science

  • ISSN

    1615-9306

  • e-ISSN

  • Volume of the periodical

    40

  • Issue of the periodical within the volume

    17

  • Country of publishing house

    DE - GERMANY

  • Number of pages

    8

  • Pages from-to

    3375-3382

  • UT code for WoS article

    000409436100001

  • EID of the result in the Scopus database

    2-s2.0-85026445642