Rapid determination of lipophilic vitamins in human serum by ultra-high performance liquid chromatography using a fluorinated column and high-throughput miniaturized liquid-liquid extraction
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F17%3A10365304" target="_blank" >RIV/00216208:11160/17:10365304 - isvavai.cz</a>
Alternative codes found
RIV/00179906:_____/17:10365304
Result on the web
<a href="http://onlinelibrary.wiley.com/doi/10.1002/jssc.201700492" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/jssc.201700492</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jssc.201700492" target="_blank" >10.1002/jssc.201700492</a>
Alternative languages
Result language
angličtina
Original language name
Rapid determination of lipophilic vitamins in human serum by ultra-high performance liquid chromatography using a fluorinated column and high-throughput miniaturized liquid-liquid extraction
Original language description
A high-throughput miniaturized liquid-liquid extraction procedure followed by a simple ultra-high performance liquid chromatography method coupled with fluorescence detection for bioanalytical analysis of all tocopherol isomers and retinol in human serumhas been developed and validated. In the extraction procedure, a synthetic internal standard tocol was used, which does not occur in the human body. The separation of structurally related vitamins was achieved using a new generation of pentafluorophenyl propyl core-shell stationary phase with elution using methanol and an aqueous solution of ammonium acetate. The fluorescence of retinol and tocopherol isomers was detected at lambda(ex) = 325, 295 nm and lambda(em) = 480, 325 nm, respectively. The rapid baseline separation of all analytes was accomplished within 4.0 min. The sensitivity of method was demonstrated with lower limits of quantification: retinol 0.01 mu M, alpha-tocopherol 0.38 mu M, beta-tocopherol 0.18 mu M, gamma-tocopherol 0.14 mu M, and delta-tocopherol 0.01 mu M. Possible application of this method in clinical practice was confirmed by the analysis of human serum samples from healthy volunteers. Finally, the simultaneous determination of retinol and all tocopherol isomers in human serum can enable the clarification of their role in metabolism and in diseases such as cancer.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30104 - Pharmacology and pharmacy
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Separation Science
ISSN
1615-9306
e-ISSN
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Volume of the periodical
40
Issue of the periodical within the volume
17
Country of publishing house
DE - GERMANY
Number of pages
8
Pages from-to
3375-3382
UT code for WoS article
000409436100001
EID of the result in the Scopus database
2-s2.0-85026445642