Determination of Retinol, alpha-Tocopherol, Lycopene, and beta-Carotene in Human Plasma Using HPLC with UV-Vis Detection: Application to a Clinical Study
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F13%3A39896326" target="_blank" >RIV/00216275:25310/13:39896326 - isvavai.cz</a>
Result on the web
<a href="http://www.hindawi.com/journals/jchem/2013/460242/" target="_blank" >http://www.hindawi.com/journals/jchem/2013/460242/</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1155/2013/460242" target="_blank" >10.1155/2013/460242</a>
Alternative languages
Result language
angličtina
Original language name
Determination of Retinol, alpha-Tocopherol, Lycopene, and beta-Carotene in Human Plasma Using HPLC with UV-Vis Detection: Application to a Clinical Study
Original language description
A method is described here for the simultaneous determination of retinol, alpha-tocopherol, lycopene, and beta-carotene in human plasma. The effectiveness of various protein precipitants and extraction solvents was tested. After adequate sample preparation, the samples were injected directed into the HPLC system. The separation was realized on analytical reversed-phase column with a UV-Vis detection. The analytical performance of this method was satisfactory. The intraassay and interaassay coefficientsof variation were below 10%. The recoveries were as follows. 97.0% (CV 2.4%) for retinol, 94.6% (CV 1.7%) for alpha-tocopherol, 91.9% (CV 3.6%) for lycopene, and 93.9% (CV 4.2%) for beta-carotene. The levels of selected fat-soluble vitamins in plasma ofpatients with cardiovascular disease were measured and discussed.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Chemistry
ISSN
2090-9063
e-ISSN
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Volume of the periodical
2013
Issue of the periodical within the volume
2013
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
"460242-1"-"460242-7"
UT code for WoS article
000311147500001
EID of the result in the Scopus database
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