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The stiffness variability of a silk fibroin scaffold during bone cell proliferation

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064165%3A_____%2F22%3A10442530" target="_blank" >RIV/00064165:_____/22:10442530 - isvavai.cz</a>

  • Alternative codes found

    RIV/46747885:24210/22:00009390 RIV/46747885:24220/22:00009390 RIV/46747885:24410/22:00009390 RIV/46747885:24620/22:00009390 RIV/00216208:11110/22:10442530

  • Result on the web

    <a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=dXWz4l_YZh" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=dXWz4l_YZh</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1177/09544119211070345" target="_blank" >10.1177/09544119211070345</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    The stiffness variability of a silk fibroin scaffold during bone cell proliferation

  • Original language description

    Complex assessment of gradual changes in scaffold morphology and stiffness is an essential step in bone filler development. Current approach, however, does not reflect long term cell proliferation effect as the mechanical tests are usually conducted on pristine materials without cells or cell influence on material stiffness is evaluated after one time period only. Here, biocompatible silk fibroin (SF) porous scaffolds envisioned for bone defect filling were prepared by dissolving of fibroin fibers, followed by dialysis, freeze-drying and final stabilization. Particular attention was devoted to the influence of bone cell proliferation up to 2 months on the stiffness of the material. The morphology of the material was studied in terms of its inner structure and the overall changes in the surface characteristics due to proliferation of MG 63 bone cell line. The SF scaffold stiffness significantly increased during first month followed by its decline during second month due to bone cell seeding. After 2 months, the SF scaffold was completely colonized, which resulted in a gradual decay of its structure. The length of degradation due to bone cell proliferation and mechanical behavior corresponded to the requirements set for reasonable filler material indicating that porous SF scaffolds comprise a promising biomaterial for bone regeneration.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    30106 - Anatomy and morphology (plant science to be 1.6)

Result continuities

  • Project

  • Continuities

    V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Others

  • Publication year

    2022

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Proceedings of the Institution of Mechanical Engineers, Part H: Journal of Engineering in Medicine

  • ISSN

    0954-4119

  • e-ISSN

    2041-3033

  • Volume of the periodical

    236

  • Issue of the periodical within the volume

    4

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    8

  • Pages from-to

    539-546

  • UT code for WoS article

    000753529300001

  • EID of the result in the Scopus database

    2-s2.0-85124912635