Three-dimensional bone tissue substitute based on a human mesenchymal stem cell culture on a nanofiber carrier and inorganic matrix

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064173%3A_____%2F16%3AN0000018" target="_blank" >RIV/00064173:_____/16:N0000018 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11120/16:43911338 RIV/62157124:16170/16:43874680

Result on the web

<a href="http://dx.doi.org/10.2754/avb201685010077" target="_blank" >http://dx.doi.org/10.2754/avb201685010077</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2754/avb201685010077" target="_blank" >10.2754/avb201685010077</a>

Result language

angličtina

Original language name

Three-dimensional bone tissue substitute based on a human mesenchymal stem cell culture on a nanofiber carrier and inorganic matrix

Original language description

The aim was to construct a composite structure for bone tissue substitute on the basis of a degradable composite of an organic nanofiber carrier and an inorganic matrix in 3D, and to achieve subsequent colonisation by differentiated human mesenchymal stem cells (hMSC) towards osteocytes. We developed an active bone tissue substitute using nanofiber technology for a polycaprolactone (PCL) scaffold with the addition of hydroxyapatite and the colonisation of both components with hMSC with the ability of differentiation towards osteocytes. The constructed composition included the components necessary for bone healing (inorganic and cellular) and it also forms a spatially-oriented 3D structure. We used polycaprolactone Mw 70,000 with electrostatic spinning for the formation of nanofibers using a modified Nanospider (TM) method. For the inorganic component we used orthophosphate-calcium silicate with a crystal size of 1-2 mm which the nanofiber membrane was coated with. Both components were connected together with a tissue adhesive based of fibrin glue. Cultivated hMSC cells at a concentration of 1.2 x 10(4)/cm(2) were multiplied in vitro and then cultivated in the expansion medium. HMSC overgrew both the PCL membrane and the Si-CaP crystals. After colonisation with cultivated cells, this composite 3D structure can serve as a three-dimensional bone tissue replacement.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FI - Traumatology and orthopaedics

OECD FORD branch

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Project

<a href="/en/project/NT14438" target="_blank" >NT14438: Development of 3-D composite bone substitution created on the basis of cultivation of osteocytes on a carrier from biopolymers processed by nanofibrous technology and anorganic matrix.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Veterinaria Brno

ISSN

0001-7213

e-ISSN

—

Volume of the periodical

85

Issue of the periodical within the volume

1

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

10

Pages from-to

77-83

UT code for WoS article

000372865100011

EID of the result in the Scopus database

2-s2.0-84962598135