DNA methylation and mRNA expression of HLA-DQA1 alleles in type 1 diabetes mellitus
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064173%3A_____%2F16%3AN0000165" target="_blank" >RIV/00064173:_____/16:N0000165 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11120/16:43911021
Result on the web
<a href="http://dx.doi.org/10.1111/imm.12593" target="_blank" >http://dx.doi.org/10.1111/imm.12593</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/imm.12593" target="_blank" >10.1111/imm.12593</a>
Alternative languages
Result language
angličtina
Original language name
DNA methylation and mRNA expression of HLA-DQA1 alleles in type 1 diabetes mellitus
Original language description
Type 1 diabetes (T1D) belongs among polygenic multifactorial autoimmune diseases. The highest risk is associated with HLA (human leukocyte antigen) class II genes, including HLA-DQA1 gene. Our aim was to investigate DNA methylation of HLA-DQA1 promoter alleles (QAP) and correlate methylation status with individual HLA-DQA1 allele expression of T1D patients and healthy controls. DNA methylation is one of the epigenetic modifications, that regulate gene expression and is known to be shaped by the environment. 61 T1D patients and 39 healthy controls were involved in this study. Isolated DNA was treated with sodium bisulfite and HLA-DQA1 promoter sequence was amplified using nested PCR. After sequencing, DNA methylation of HLA-DQA1 promoter alleles was analyzed. Individual mRNA HLA-DQA1 relative allele expression was assessed using two different endogenous controls (PPIA, DRA). We have found statistically significant differences in HLA-DQA1 allele 02:01 expression (PPIA normalization, Pcorr =0.041; DRA normalization, Pcorr =0.052) between healthy controls and T1D patients. The complete methylation profile of the HLA-DQA1 promoter was gained with the most methylated allele DQA1*02:01 and the least methylated DQA1*05:01 in both studied groups. Methylation profile observed in T1D patients and healthy controls was similar, and no correlation between HLA-DQA1 allele expression and DNA methylation was found. Although we have not proved significant methylation differences between the two groups, detailed DNA methylation status and its correlation with expression of each HLA-DQA1 allele in T1D patients have been described for the first time.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Immunology
ISSN
0019-2805
e-ISSN
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Volume of the periodical
148
Issue of the periodical within the volume
2
Country of publishing house
GB - UNITED KINGDOM
Number of pages
10
Pages from-to
150-159
UT code for WoS article
000381284200004
EID of the result in the Scopus database
2-s2.0-84960155903