Differences in promoter DNA methylation and mRNA expression of individual alleles of the HLA class II DQA1 gene

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F15%3A43910218" target="_blank" >RIV/00216208:11120/15:43910218 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.imlet.2015.08.006" target="_blank" >http://dx.doi.org/10.1016/j.imlet.2015.08.006</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.imlet.2015.08.006" target="_blank" >10.1016/j.imlet.2015.08.006</a>

Result language

angličtina

Original language name

Differences in promoter DNA methylation and mRNA expression of individual alleles of the HLA class II DQA1 gene

Original language description

Objectives: Extensive polymorphism of HLA class II genes is not restricted to the coding region of the gene. It extends also to the linked promoter region, where it forms the basis for different levels of individual allele's expression. Differential expression of HLA class II alleles can shape an immune response and influence the risk of developing autoimmune disease. In addition to genetic variability, variation in epigenetic modifications, including DNA methylation, can be another cause of the unevenexpression of individual alleles. We aimed to analyze the DNA methylation of promoter sequences and the levels of expression of individual DQA1 gene alleles, interallelic variation of these two characteristics and the relationship between them. Methods:The 60 healthy donors included into study were HLA-DRB1, HLA-DQB1 and HLA-DQA1 genotyped using PCR-SSP. Genomic DNA was treated by sodium bisulfite and the target segment in the HLA-DQA1 gene promoter was PCR amplified. PCR product was cl

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EC - Immunology

OECD FORD branch

—

Project

—

Continuities

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Immunology Letters

ISSN

0165-2478

e-ISSN

—

Volume of the periodical

167

Issue of the periodical within the volume

2

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

8

Pages from-to

147-154

UT code for WoS article

000362628200012

EID of the result in the Scopus database

—