Enrichment of circulating trophoblasts from maternal blood using filtration-based Metacell(R) technology
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064173%3A_____%2F22%3A43923718" target="_blank" >RIV/00064173:_____/22:43923718 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1371/journal.pone.0271226" target="_blank" >https://doi.org/10.1371/journal.pone.0271226</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1371/journal.pone.0271226" target="_blank" >10.1371/journal.pone.0271226</a>
Alternative languages
Result language
angličtina
Original language name
Enrichment of circulating trophoblasts from maternal blood using filtration-based Metacell(R) technology
Original language description
In a cell-based non-invasive prenatal test (cbNIPT), intact circulating trophoblasts (CTs) are isolated from maternal blood for subsequent genetic analysis. Enrichment of these CTs from maternal blood is the most challenging step in the cbNIPT workflow. This study aims to assess the suitability of the filtration-based Metacell(R) technology to enrich CTs from maternal blood at week 10 to 13 of gestation. The Metacell(R) technology is a novel size-based enrichment technology that combines blood filtration through 8 μm pores with an in vitro culture method. Three protocols were evaluated. First, 8 mL or 16 mL of maternal blood was filtered and subsequently cultured in vitro on the separation membrane for 3 days in RPMI 1640. In addition, 16 mL of maternal blood was filtered, and immediately processed without further culturing. Y-chromosome-specific qPCR or STR analysis was performed to evaluate the enrichment of CTs. A total of 44 samples from pregnant women, out of which 26 were carrying a male fetus, were processed. Although five enriched male fetus samples show detectable male DNA quantities, it cannot be excluded that the obtained positive signal is caused by cell-free fetal DNA sticking to the Metacell(R) separation membrane. In conclusion, the Metacell(R) technology, tested as described, is not suitable for consistent enrichment of CTs.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30101 - Human genetics
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
PLoS One
ISSN
1932-6203
e-ISSN
1932-6203
Volume of the periodical
17
Issue of the periodical within the volume
7
Country of publishing house
US - UNITED STATES
Number of pages
14
Pages from-to
"e0271226"
UT code for WoS article
000922616700001
EID of the result in the Scopus database
2-s2.0-85134166331