Assessment of Faecal Microbiota Transplant Stability in Deep-Freeze Conditions: A 12-Month Ex Vivo Viability Analysis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064173%3A_____%2F24%3A43926888" target="_blank" >RIV/00064173:_____/24:43926888 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11120/24:43926888
Result on the web
<a href="https://doi.org/10.1002/jcla.25023" target="_blank" >https://doi.org/10.1002/jcla.25023</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jcla.25023" target="_blank" >10.1002/jcla.25023</a>
Alternative languages
Result language
angličtina
Original language name
Assessment of Faecal Microbiota Transplant Stability in Deep-Freeze Conditions: A 12-Month Ex Vivo Viability Analysis
Original language description
Background: Faecal microbiota transplantation (FMT) is an established treatment for Clostridioides difficile infection and is under investigation for other conditions. The availability of suitable donors and the logistics of fresh stool preparation present challenges, making frozen, biobanked stools an attractive alternative. Aims: This study aimed to evaluate the long-term viability of bacterial populations in faecal samples stored at -80oC for up to 12 months, supporting the feasibility of using frozen grafts for FMT. Methods: Fifteen faecal samples from nine healthy donors were processed, mixed with cryoprotectants and stored at -80oC. Samples were assessed at baseline and after 3, 6 and 12 months using quantitative culturing methods to determine the concentration of live bacteria. Results: Quantitative analysis showed no significant decrease in bacterial viability over the 12-month period for both aerobic and anaerobic cultures (p = 0.09). At all timepoints, the coefficients of variability in colony-forming unit (CFU) counts were greater between samples (102 +- 21% and 100 +- 13% for aerobic and anaerobic cultures, respectively) than the variability between measurements of the same sample (30 +- 22% and 30 +- 19%). Conclusions: The study confirmed that faecal microbiota can be preserved with high viability in deep-freeze storage for up to a year, making allogenic FMT from biobanked samples a viable and safer option for patients. However, a multidonor approach may be beneficial to mitigate the risk of viability loss in any single donor sample.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30221 - Critical care medicine and Emergency medicine
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Clinical Laboratory Analysis
ISSN
0887-8013
e-ISSN
1098-2825
Volume of the periodical
38
Issue of the periodical within the volume
7
Country of publishing house
US - UNITED STATES
Number of pages
5
Pages from-to
"e25023"
UT code for WoS article
001191997800001
EID of the result in the Scopus database
2-s2.0-85189533145