Contribution of glucose and glutamine to hypoxia-induced lipid synthesis decreases, while contribution of acetate increases, during 3T3-L1 differentiation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064173%3A_____%2F24%3A43927736" target="_blank" >RIV/00064173:_____/24:43927736 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/24:10487590 RIV/00216208:11120/24:43927736 RIV/00216208:11310/24:10487590 RIV/00064190:_____/24:10001288 RIV/00064165:_____/24:10487590

Result on the web

<a href="https://doi.org/10.1038/s41598-024-79458-0" target="_blank" >https://doi.org/10.1038/s41598-024-79458-0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-024-79458-0" target="_blank" >10.1038/s41598-024-79458-0</a>

Result language

angličtina

Original language name

Contribution of glucose and glutamine to hypoxia-induced lipid synthesis decreases, while contribution of acetate increases, during 3T3-L1 differentiation

Original language description

The molecular mechanisms linking obstructive sleep apnea syndrome (OSA) to obesity and the development of metabolic diseases are still poorly understood. The role of hypoxia (a characteristic feature of OSA) in excessive fat accumulation has been proposed. The present study investigated the possible effects of hypoxia (4% oxygen) on de novo lipogenesis by tracking the major carbon sources in differentiating 3T3-L1 adipocytes. Gas-permeable cultuware was employed to cultivate 3T3-L1 adipocytes in hypoxia (4%) for 7 or 14 days of differentiation. We investigated the contribution of glutamine, glucose or acetate using (13)C or (14)C labelled carbons to the newly synthesized lipid pool, changes in intracellular lipid content after inhibiting citrate- or acetate-dependent pathways and gene expression of involved key enzymes. The results demonstrate that, in differentiating adipocytes, hypoxia decreased the synthesis of lipids from glucose (44.1 +- 8.8 to 27.5 +- 3.0 pmol/mg of protein, p &lt; 0.01) and partially decreased the contribution of glutamine metabolized through the reverse tricarboxylic acid cycle (4.6% +- 0.2-4.2% +- 0.1%, p &lt; 0.01). Conversely, the contribution of acetate, a citrate- and mitochondria-independent source of carbons, increased upon hypoxia (356.5 +- 71.4 to 649.8 +- 117.5 pmol/mg of protein, p &lt; 0.01). Further, inhibiting the citrate- or acetate-dependent pathways decreased the intracellular lipid content by 58% and 73%, respectively (p &lt; 0.01) showing the importance of de novo lipogenesis in hypoxia-exposed adipocytes. Altogether, hypoxia modified the utilization of carbon sources, leading to alterations in de novo lipogenesis in differentiating adipocytes and increased intracellular lipid content.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30202 - Endocrinology and metabolism (including diabetes, hormones)

Project

<a href="/en/project/NU21-01-00259" target="_blank" >NU21-01-00259: Novel inhibitors of lipolysis in the treatment of lipid a glucose metabolism in obstructive sleep apnea syndrome</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Scientific Reports

ISSN

2045-2322

e-ISSN

2045-2322

Volume of the periodical

14

Issue of the periodical within the volume

November

Country of publishing house

GB - UNITED KINGDOM

Number of pages

10

Pages from-to

28193

UT code for WoS article

001356230100037

EID of the result in the Scopus database

2-s2.0-85209079353