Intermittent Hypoxia Stimulates Lipolysis, But Inhibits Differentiation and De Novo Lipogenesis in 3T3-L1 Cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F20%3A43919629" target="_blank" >RIV/00216208:11120/20:43919629 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/20:10403740 RIV/75010330:_____/20:00012984
Result on the web
<a href="https://doi.org/10.1089/met.2019.0112" target="_blank" >https://doi.org/10.1089/met.2019.0112</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1089/met.2019.0112" target="_blank" >10.1089/met.2019.0112</a>
Alternative languages
Result language
angličtina
Original language name
Intermittent Hypoxia Stimulates Lipolysis, But Inhibits Differentiation and De Novo Lipogenesis in 3T3-L1 Cells
Original language description
Exposure to intermittent hypoxia (IH) may play a role in the development of metabolic impairments in the context of obstructive sleep apnea syndrome, probably by elevated plasma levels of free fatty acids. Employing gas-permeable cultureware to grow differentiated human and mouse adipocytes , we directly studied the effects of pericellular oxygen fluctuations on key adipocyte metabolic functions-spontaneous lipolytic rates, triglyceride accumulation, lipogenesis, and expression of adipocyte-specific marker genes. 3T3-L1 fibroblasts and human subcutaneous preadipocytes were differentiated under conditions that induced repetitive pericellular-oxygen cycles IH between 1% O (5 min) and 16% O (5 min), continuously for 14 days or under control conditions. Chemicals were used to inhibit the flux of acetyl-CoA from glycolysis (alfa-cyano-4-hydroxy cinnamate) or the tricarboxylic acid cycle (SB204990), or to stimulate the flux of acetyl-CoA from pyruvate to the lipogenic pool. Lipolytic rate, intracellular lipids, and expression of adipocyte differentiation markers were assessed and -test or ANOVA were used to find significant differences. The rate of lipolysis increased by 211% in 3T3-L1 cells and by 39% in obese human adipocytes. Exposure to IH reduced intracellular lipid stores by 37% and reduced the expression of adipocyte differentiation markers. Pharmacological stimulation or inhibition of lipogenesis did not modify the intracellular lipid content under IH. Pericellular oxygen fluctuations directly stimulated lipolysis, but did not increase lipogenesis from endogenous substrates. Similarly, IH hampered adipocyte differentiation from precursors.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30202 - Endocrinology and metabolism (including diabetes, hormones)
Result continuities
Project
<a href="/en/project/GA18-10144S" target="_blank" >GA18-10144S: Hypoxia-induced Mitochondrial Adaptations as the Unifying Factor of Type 2 Diabetes Development in Sleep Apnea Syndrome</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Metabolic Syndrome and Related Disorders
ISSN
1540-4196
e-ISSN
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Volume of the periodical
18
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
146-153
UT code for WoS article
000507270000001
EID of the result in the Scopus database
2-s2.0-85083041201