Different Expression of Vascularization and Inflammatory Regulators in Cells Derived from Oral Mucosa and Limbus
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064173%3A_____%2F25%3A43928685" target="_blank" >RIV/00064173:_____/25:43928685 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11110/25:10500157 RIV/00216208:11120/25:43928685
Result on the web
<a href="https://doi.org/10.3390/bioengineering12070688" target="_blank" >https://doi.org/10.3390/bioengineering12070688</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/bioengineering12070688" target="_blank" >10.3390/bioengineering12070688</a>
Alternative languages
Result language
angličtina
Original language name
Different Expression of Vascularization and Inflammatory Regulators in Cells Derived from Oral Mucosa and Limbus
Original language description
Bilateral limbal stem cell deficiency (LSCD) can be effectively treated with cultivated oral mucosa epithelial cell transplantation (COMET). However, COMET is associated with greater superficial neovascularization than limbal stem cell (LESC) transplantation, the gold standard for unilateral LSCD. To investigate the intrinsic molecular features of cells intended for grafting, we assessed the in vitro expression of genes involved in vascularization and inflammation using real-time quantitative PCR and multifactorial linear models. Oral mucosal epithelial cells (OMECs) and limbal epithelial cells (LECs) were cultured in either conventional (COM) or xenobiotic-free (XF) media on fibrin substrates. Gene expression profiling revealed distinct transcriptional signatures. The pro-angiogenic genes AGR2, ANGPTL2, CRYAB, EREG, JAM3, and S100A4 were significantly higher in LECs (adjusted p < 0.01), whereas FGF2 was higher in OMECs (adjusted p < 0.001). The anti-angiogenic genes TIMP3 and SERPINF1 were higher in LECs (adjusted p < 0.01), while COL18A1 was higher in OMECs (adjusted p < 0.01). OMECs also showed significantly greater expression of the immunoregulatory genes IL1B, IL6, TNF, CXCL10, and IL1RN (adjusted p < 0.01). Cultivation induced phenotypic changes in OMECs, with COM and XF media exerting comparable effects. These results highlight the contribution of inflammatory mediators to neovascularization following COMET.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30207 - Ophthalmology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Bioengineering
ISSN
2306-5354
e-ISSN
2306-5354
Volume of the periodical
12
Issue of the periodical within the volume
7
Country of publishing house
CH - SWITZERLAND
Number of pages
14
Pages from-to
688
UT code for WoS article
001536701600001
EID of the result in the Scopus database
2-s2.0-105011770139