Iron Must Be in Haemin to Act as a Pro-Inflammatory Stimulus in Cultured Human THP-1 Monocytes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064173%3A_____%2F25%3A43929273" target="_blank" >RIV/00064173:_____/25:43929273 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/25:10507730 RIV/00216208:11120/25:43929273

Result on the web

<a href="https://doi.org/10.14712/fb2025.0001" target="_blank" >https://doi.org/10.14712/fb2025.0001</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.14712/fb2025.0001" target="_blank" >10.14712/fb2025.0001</a>

Result language

angličtina

Original language name

Iron Must Be in Haemin to Act as a Pro-Inflammatory Stimulus in Cultured Human THP-1 Monocytes

Original language description

Cardiovascular diseases due to atherosclerosis remain a dominant medical problem. Macro-phages play a crucial role in both atherosclerosis progression and recycling of body iron. Epidemio-logical data point to elevation of body iron stores as one of non-classical cardiovascular risk factors, and we know that iron must be contained within macrophages to be atherogenic. Presumably, iron already contained within circulating monocytes turns cells to a more pro-inflammatory and hence atherogenic phenotype, but experimental evidence for such relationship remains limited. In this study, human monocytic THP-1 cells were loaded with micromolar iron in the forms of transferrin, ferric-ammonium citrate (FAC) and haemin for 2 and 24 hours. Only haemin was cytotoxic. All kinds of iron elevated the labile iron pool at 2 hours, as well as ferritin expression at 24 hours. Expression of scavenger receptors A and B, pro-inflammatory as well as anti-inflammatory cytokines, haem oxygenase and haptoglobin receptor were measured by quantitative PCR. Very few changes, none pro-inflammatory, were observed in response to transferrin or FAC. Haemin suppressed the expression of scavenger receptors, increased the expression of pro-inflammatory, and variably, anti-inflammatory cytokines, and dramatically induced haem oxygenase. The effects of haemin were not prevented by apotransferrin. We conclude that iron must be in the haemin form to act as a pro-inflammatory stimulus in THP-1 monocytes. Non-haem iron might require the presence of other factors to be atherogenic. Haemin treatment of THP-1 cells may represent a convenient experimental model to study the pro-inflammatory effects of haem that are observed in late stages of atherosclerosis.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30202 - Endocrinology and metabolism (including diabetes, hormones)

Project

<a href="/en/project/NT13671" target="_blank" >NT13671: Intracellular reactive iron in monocytes and its role in atherogenesis</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Folia Biologica

ISSN

0015-5500

e-ISSN

2533-7602

Volume of the periodical

71

Issue of the periodical within the volume

4

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

13

Pages from-to

149-161

UT code for WoS article

001669101300001

EID of the result in the Scopus database

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