Glutathione peroxidase 3 localizes to the epithelial lining fluid and the extracellular matrix in interstitial lung disease

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064190%3A_____%2F16%3AN0000015" target="_blank" >RIV/00064190:_____/16:N0000015 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/16:10327505

Result on the web

<a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4951690/pdf/srep29952.pdf" target="_blank" >https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4951690/pdf/srep29952.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/srep29952" target="_blank" >10.1038/srep29952</a>

Result language

angličtina

Original language name

Glutathione peroxidase 3 localizes to the epithelial lining fluid and the extracellular matrix in interstitial lung disease

Original language description

Aberrant antioxidant activity and excessive deposition of extracellular matrix (ECM) are hallmarks of interstitial lung diseases (ILD). It is known that oxidative stress alters the ECM, but extracellular antioxidant defence mechanisms in ILD are incompletely understood. Here, we extracted abundance and detergent solubility of extracellular antioxidant enzymes from a proteomic dataset of bleomycin-induced lung fibrosis in mice and assessed regulation and distribution of glutathione peroxidase 3 (GPX3) in murine and human lung fibrosis. Superoxide dismutase 3 (Sod3), Gpx3, and Gpx activity were increased in mouse BALF during bleomycin-induced lung fibrosis. In lung tissue homogenates, Gpx3, but not Sod3, was upregulated and detergent solubility profiling indicated that Gpx3 associated with ECM proteins. Immunofluorescence analysis showed that Gpx3 was expressed by bronchial epithelial cells and interstitial fibroblasts and localized to the basement membrane and interstitial ECM in lung tissue. As to human ILD samples, BALF of some patients contained high levels of GPX3, and GPX3 was upregulated in lung homogenates from IPF patients. GPX3 expression in primary human bronchial epithelial cells and lung fibroblasts was downregulated by TNF-alpha, but more variably regulated by TGF-beta 1 and menadione. In conclusion, the antioxidant enzyme GPX3 localizes to lung ECM and is variably upregulated in ILD.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FC - Pneumology

OECD FORD branch

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Project

<a href="/en/project/NT13433" target="_blank" >NT13433: Immuno-pathogenetic backing data for the fibro-genesis for interstitial pulmonary processes, biomarkers of fibro-genesis</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

SCIENTIFIC REPORTS

ISSN

2045-2322

e-ISSN

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Volume of the periodical

Neuveden

Issue of the periodical within the volume

6

Country of publishing house

GB - UNITED KINGDOM

Number of pages

15

Pages from-to

nestránkováno

UT code for WoS article

000379924000002

EID of the result in the Scopus database

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