The mechanism of cytotoxicity and DNA adduct formation by the anticancer drug ellipticine in human neuroblastoma cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064203%3A_____%2F09%3A5287" target="_blank" >RIV/00064203:_____/09:5287 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/09:10001346 RIV/00216208:11130/09:5287
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
The mechanism of cytotoxicity and DNA adduct formation by the anticancer drug ellipticine in human neuroblastoma cells
Original language description
The mechanism of ellipticine action in human neuroblastoma IMR-32, UKF-NB-3 and UKF-NB-4 cell lines was investigated. Treatment of neuroblastoma with ellipticine resulted in apoptosis induction. This effect was associated with formation of covalent ellipticine-derived DNA adducts, identical to those formed by the cytochrome P450- and peroxidase-mediated ellipticine metabolites. The expression of these enzymes and their ability to generate ellipticine-DNA adducts in neuroblastoma were proven. The levelsof DNA adducts correlated with toxicity of ellipticine to IMR-32 and UKF-NB-4 cells, but not to UKF-NB-3. In addition, hypoxic conditions resulted in decrease in ellipticine toxicity and correlated with lower levels of adducts. Both these lines accumulated in S phase, suggesting that adducts interfere with DNA replication. The results demonstrate that among modes of ellipticine antitumor action, formation of adducts is the main mechanism of cytotoxicity to neuroblastoma.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FD - Oncology and haematology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biochemical Pharmacology
ISSN
0006-2952
e-ISSN
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Volume of the periodical
77
Issue of the periodical within the volume
9
Country of publishing house
US - UNITED STATES
Number of pages
14
Pages from-to
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UT code for WoS article
000265197000003
EID of the result in the Scopus database
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