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The mechanism of cytotoxicity and DNA adduct formation by the anticancer drug ellipticine in human neuroblastoma cells

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064203%3A_____%2F09%3A5287" target="_blank" >RIV/00064203:_____/09:5287 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216208:11310/09:10001346 RIV/00216208:11130/09:5287

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    The mechanism of cytotoxicity and DNA adduct formation by the anticancer drug ellipticine in human neuroblastoma cells

  • Original language description

    The mechanism of ellipticine action in human neuroblastoma IMR-32, UKF-NB-3 and UKF-NB-4 cell lines was investigated. Treatment of neuroblastoma with ellipticine resulted in apoptosis induction. This effect was associated with formation of covalent ellipticine-derived DNA adducts, identical to those formed by the cytochrome P450- and peroxidase-mediated ellipticine metabolites. The expression of these enzymes and their ability to generate ellipticine-DNA adducts in neuroblastoma were proven. The levelsof DNA adducts correlated with toxicity of ellipticine to IMR-32 and UKF-NB-4 cells, but not to UKF-NB-3. In addition, hypoxic conditions resulted in decrease in ellipticine toxicity and correlated with lower levels of adducts. Both these lines accumulated in S phase, suggesting that adducts interfere with DNA replication. The results demonstrate that among modes of ellipticine antitumor action, formation of adducts is the main mechanism of cytotoxicity to neuroblastoma.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    FD - Oncology and haematology

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2009

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Biochemical Pharmacology

  • ISSN

    0006-2952

  • e-ISSN

  • Volume of the periodical

    77

  • Issue of the periodical within the volume

    9

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    14

  • Pages from-to

  • UT code for WoS article

    000265197000003

  • EID of the result in the Scopus database