Multiplexed immuno-precipitation with 1725 commercially available antibodies to cellular proteins

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064203%3A_____%2F11%3A7143" target="_blank" >RIV/00064203:_____/11:7143 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11130/11:7143

Result on the web

<a href="http://www.ncbi.nlm.nih.gov/pubmed/21956872" target="_blank" >http://www.ncbi.nlm.nih.gov/pubmed/21956872</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Multiplexed immuno-precipitation with 1725 commercially available antibodies to cellular proteins

Original language description

Antibody array analysis of complex samples requires capture reagents with exceptional specificity. The frequency of antibodies with label-based detection may be as low as 5%. Here, however, we show that as many as 25% of commercially available antibodiesare useful when biotinylated cellular proteins are fractionated by size exclusion chromatography (SEC) first. A microsphere multiplex with 1725 antibodies to cellular proteins was added to 24 SEC fractions, labelled with streptavidin and analyzed by flow cytometry (microsphere-based affinity proteomics, MAP) The SEC-MAP approach resolved different targets captured by each antibody as reactivity peaks across the separation range of the SEC column (10-670kDa). Complex reactivity profiles demonstrated that most antibodies bound more than one target. However, specific binding was readily detected as reactivity peaks common for different antibodies to the same protein. We optimized sample preparation and found that amine-reactive biotin rar

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FD - Oncology and haematology

OECD FORD branch

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Project

<a href="/en/project/7F09102" target="_blank" >7F09102: EEA/Norwegian Financial Mechanisms and Ministry of education, Youth and Sports of Czech republic</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Proteomics

ISSN

1615-9853

e-ISSN

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Volume of the periodical

11

Issue of the periodical within the volume

23

Country of publishing house

DE - GERMANY

Number of pages

5

Pages from-to

4578-4582

UT code for WoS article

000297582600013

EID of the result in the Scopus database

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