PCR detection of Burkholderia cepacia complex as one of key factors to handle a long-term outbreak

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064203%3A_____%2F12%3A8263" target="_blank" >RIV/00064203:_____/12:8263 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11130/12:8263
Result on the web
<a href="http://dx.doi.org/10.1016/j.jcf.2012.04.005" target="_blank" >http://dx.doi.org/10.1016/j.jcf.2012.04.005</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
PCR detection of Burkholderia cepacia complex as one of key factors to handle a long-term outbreak
Original language description
Background: Once the outbreak with Burkholderia cenocepacia ST32 was identified in the Prague cystic fibrosis (CF) centre, molecular tools were implemented into diagnostic routine in order to complement infection control measures with as accurate as possible microbiological service. In addition, genotyping techniques were applied as part of an infection surveillance program to assign species and strain status in samples positive for Burkholderia cepacia complex (Bcc). We sought to evaluate a usefulnessof Bcc-specific PCR in infection control and to map evolution of the outbreak. Methods: Since 2001, 6109 respiratory samples from 299 CF patients were examined not only by conventional culture, but also by PCR, detecting Bcc directly in sputum. Results:Diagnosis of Bcc infection was established by culture in 54 patients already prior to 2001. As 39 more patients were diagnosed by culture and/or PCR during 2001-2010, this represented annual prevalence of 18.5%-28.9%. Twelve of 39 patient
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FC - Pneumology
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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