Determination of Urine Albumin by New Simple High-Performance Liquid Chromatography Method
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064203%3A_____%2F16%3A10332570" target="_blank" >RIV/00064203:_____/16:10332570 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11130/16:10332570
Result on the web
<a href="http://dx.doi.org/10.1002/jcla.22007" target="_blank" >http://dx.doi.org/10.1002/jcla.22007</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jcla.22007" target="_blank" >10.1002/jcla.22007</a>
Alternative languages
Result language
angličtina
Original language name
Determination of Urine Albumin by New Simple High-Performance Liquid Chromatography Method
Original language description
BackgroundA simple high-performance liquid chromatography (HPLC) method was developed for the determination of albumin in patients' urine samples without coeluting proteins and was compared with the immunoturbidimetric determination of albumin. Urine albumin is important biomarker in diabetic patients, but part of it is immuno-nonreactive. MethodsAlbumin was determined by high-performance liquid chromatography (HPLC), UV detection at 280 nm, Zorbax 300SB-C3 column. Immunoturbidimetric analysis was performed using commercial kit on automatic biochemistry analyzer COBAS INTEGRA((R)) 400, Roche Diagnostics GmbH, Manheim, Germany. ResultsThe HLPC method was fully validated. No significant interference with other proteins (transferrin, -1-acid glycoprotein, -1-antichymotrypsin, antitrypsin, hemopexin) was found. The results from 301 urine samples were compared with immunochemical determination. We found a statistically significant difference between these methods (P = 0.0001, Mann-Whitney test). ConclusionNew simple HPLC method was developed for the determination of urine albumin without coeluting proteins. Our data indicate that the HPLC method is highly specific and more sensitive than immunoturbidimetry.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Clinical Laboratory Analysis
ISSN
0887-8013
e-ISSN
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Volume of the periodical
30
Issue of the periodical within the volume
6
Country of publishing house
US - UNITED STATES
Number of pages
6
Pages from-to
1226-1231
UT code for WoS article
000386935200069
EID of the result in the Scopus database
2-s2.0-84973621032