Estimated Total Albumin in Fresh Urine Samples Based on Correlation Between the Roche Immunoturbidimetric and an In-House HPLC Method
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F18%3A10387163" target="_blank" >RIV/00216208:11130/18:10387163 - isvavai.cz</a>
Alternative codes found
RIV/00064203:_____/18:10387163
Result on the web
<a href="https://doi.org/10.7754/Clin.Lab.2018.180410" target="_blank" >https://doi.org/10.7754/Clin.Lab.2018.180410</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.7754/Clin.Lab.2018.180410" target="_blank" >10.7754/Clin.Lab.2018.180410</a>
Alternative languages
Result language
angličtina
Original language name
Estimated Total Albumin in Fresh Urine Samples Based on Correlation Between the Roche Immunoturbidimetric and an In-House HPLC Method
Original language description
Background: Many studies have reported higher values of urinary albumin measured by high performance liquid chromatography (HPLC) in comparison with immunochemical methods. The aims of our study were the implementation of the HPLC method for albuminuria, testing the hypothesis about coeluting proteins, comparison of albuminuria assessed by HPLC and immunoturbidimetric (IT) methods in diabetic and non-diabetic patient samples. Methods: We compared albuminuria assessed by HPLC with albuminuria assessed by the IT method in fresh urine samples of 636 diabetics and 456 non-diabetics. We investigated relationships between albuminuria and blood glycated hemoglobin HbA(1c). Results: We found significant differences between the parameters of linear regressions between albuminuria determined using HPLC and IT among patients with and without DM, and even between patients with DM type 1 and type 2. We confirmed the underestimation of albuminuria assessed by IT. We did not reveal any significant correlation between blood glycated hemoglobin and any of the parameters derived from albuminuria. Conclusions: We excluded non-specificity of the HPLC method. Despite of a little bit lower analytical sensitivity of the HPLC method in comparison with IT method the diagnostic sensitivity of HPLC method is higher, because it measures the total albuminuria (immunoreactive plus immuno-unreactive). We developed three formulas (for nondiabetics, for diabetics type 1 and diabetics type 2) for the estimation of the total albuminuria from IT values. We also confirmed that albuminuria and HbA(1c) are independent biomarkers.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Clinical Laboratory
ISSN
1433-6510
e-ISSN
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Volume of the periodical
64
Issue of the periodical within the volume
11-12
Country of publishing house
DE - GERMANY
Number of pages
11
Pages from-to
1835-1845
UT code for WoS article
000452882500006
EID of the result in the Scopus database
2-s2.0-85058607954