LC-MS/MS quantitative analysis of phylloquinone, menaquinone-4 and menaquinone-7 in the human serum of a healthy population
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064203%3A_____%2F19%3A10398456" target="_blank" >RIV/00064203:_____/19:10398456 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11130/19:10398456
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=EoRVlEj9Ph" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=EoRVlEj9Ph</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.7717/peerj.7695" target="_blank" >10.7717/peerj.7695</a>
Alternative languages
Result language
angličtina
Original language name
LC-MS/MS quantitative analysis of phylloquinone, menaquinone-4 and menaquinone-7 in the human serum of a healthy population
Original language description
A novel application of the liquid chromatography method combined with the triple quadrupole tandem mass spectrometry method was developed for the quantification of vitamin K-1 and two forms of vitamin K-2 (menaquinone-4, menaquinone-7) in human serum. Total chromatography time for each run was 9 min. Time required for the sample pretreatment procedures was approximately 4 h. The coefficients of variation (CVs) of intra-assay were 10.4%, 3.2 % and 2.3% for vitamin K-1 in three levels of quality control samples; were 14.3%, 3.2% and 6.7% for menaquinone-4; and were 11.1%, 6.0% and 7.0% for menaquinone-7. The inter-assay CVs were 12.8%, 11.3% and 7.4% for vitamin K-1; were 15.2%, 9.2% and 8.7% for menaquinone-4; and were 13.2%, 11.1% and 7.2% for menaquinone-7. No interference was found between K-1, menaquinone-4 and menaquinone-7, nor any deuterated internal standards. This method was then used to determine reference values for Caucasian populations of central European origin. Samples were measured from 191 healthy volunteers (51.2 +/- 16.2 years (mean +/- SD)) and the values concerning K-1 were 0.044-1.357 ng/mL for women and 0.030-1.214 ng/mL for men. The values for menaquinone-4 and menaquinone-7 did not exhibit any differences between women and men, and were 0.050-1.598 and 0.074-0.759 ng/mL, respectively.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
PeerJ
ISSN
2167-8359
e-ISSN
—
Volume of the periodical
7
Issue of the periodical within the volume
September
Country of publishing house
US - UNITED STATES
Number of pages
14
Pages from-to
e7695
UT code for WoS article
000486566300008
EID of the result in the Scopus database
2-s2.0-85074108243