Determination of vitamins K-1, MK-4, and MK-7 in human serum of postmenopausal women by HPLC with fluorescence detection

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F18%3A10376155" target="_blank" >RIV/00216208:11130/18:10376155 - isvavai.cz</a>

Alternative codes found

RIV/00064203:_____/18:10376155

Result on the web

<a href="https://doi.org/10.1002/jcla.22381" target="_blank" >https://doi.org/10.1002/jcla.22381</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jcla.22381" target="_blank" >10.1002/jcla.22381</a>

Result language

angličtina

Original language name

Determination of vitamins K-1, MK-4, and MK-7 in human serum of postmenopausal women by HPLC with fluorescence detection

Original language description

BackgroundNew high-performance liquid chromatography (HPLC) method was developed for the determination of vitamin K-1 and two forms of vitamin K-2 (MK-4 and MK-7) in human serum, and the levels of vitamin Kwere determined in 350 samples of postmenopausal women. MethodsVitamin K was determined by HPLC with fluorescence detection after postcolumn zinc reduction. The detection was performed at 246nm (excitation) and 430nm (emission). The internal standard and 2mL of ethanol were added to 500L of serum. The mixture was extracted with 4mL of hexane, and solid phase extraction was then used. ResultsThe HLPC method was fully validated. The intra- and interday accuracy and precision were evaluated on two QC samples by multiple analysis, and CV were less than 10%. The limit of quantification for MK-4 was found at 0.04 ng/mL, for K-1 0.03ng/mL, and for MK-7 0.03ng/mL. The mean recoveries of the corresponding compounds were 98%-110%. Serum levels of MK-4, K-1, and MK-7 in postmenopausal women with osteoporosis were 0.8900.291ng/mL, 0.433 +/- 0.394ng/mL, and 1.002 +/- 1.020ng/mL, respectively (mean +/- SD). Serum levels of MK-4, K-1, and MK-7 in postmenopausal women without osteoporosis were 0.825 +/- 0.266ng/mL, 0.493 +/- 0.399ng/mL, and 1.186 +/- 1.076ng/mL, respectively (mean +/- SD). ConclusionNew HPLC method for the determination of vitamins K-1, MK-4, and MK-7 in serum was evaluated and validated. This method is highly specific and sensitive with the low limit of quantification.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Clinical Laboratory Analysis

ISSN

0887-8013

e-ISSN

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Volume of the periodical

32

Issue of the periodical within the volume

5

Country of publishing house

US - UNITED STATES

Number of pages

6

Pages from-to

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UT code for WoS article

000435263900017

EID of the result in the Scopus database

2-s2.0-85048559977