Analysis of vitamin K1 and major K2 variants in rat/human serum and lipoprotein fractions by a rapid simple, and sensitive UHPLC-MS/MS methods

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F24%3A10476184" target="_blank" >RIV/00216208:11160/24:10476184 - isvavai.cz</a>

Alternative codes found

RIV/00179906:_____/24:10476184

Result on the web

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=p9jj5XbJxB" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=p9jj5XbJxB</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2023.464548" target="_blank" >10.1016/j.chroma.2023.464548</a>

Result language

angličtina

Original language name

Analysis of vitamin K1 and major K2 variants in rat/human serum and lipoprotein fractions by a rapid simple, and sensitive UHPLC-MS/MS methods

Original language description

Determination of the various forms of vitamin K, which are involved in coagulation and other physiological processes in humans, is challenging and no standardized method is yet available. Therefore, a reliable and practical method was developed to quantify vitamin K levels in serum and additionally in lipoprotein fractions to clarify its distribution. The LC-MS/MS method for the determination of vitamin K1 and the three main isoforms of vitamin K2 (MK-4, MK-7, MK-9) was combined with a gradient ultracentrifugation technique to allow the separation of lipoprotein fractions. The chromatographic separation was carried out on a KinetexTM C18 column using a mobile phase consisting mainly of methanol. The target analytes were detected by electrospray ionization mass spectrometry. The separation of all four substances was achieved after a simple sample preparation technique based on miniaturized liquid-liquid extraction. Our method of only 8.5 min revealed the levels of the major forms of vitamin K in 59 human and 12 rat sera and confirmed our hypothesis that vitamin K is primarily (about 50 %) found in the high-density lipoprotein fraction. The median concentrations of vitamin K1, MK-4, MK-7, and MK-9 were found to be 1.19, 2.98, 0.43, and &lt; 0.71 nmol/L in human serum and 1.74, 6.75, less than 0.2, and less than 0.5 nmol/L in rat serum, respectively.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30104 - Pharmacology and pharmacy

Project

<a href="/en/project/NU21J-02-00021" target="_blank" >NU21J-02-00021: Differences in parameters of platelets aggregation and blood coagulation between healthy individuals and patients with metabolic diseases</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

1873-3778

Volume of the periodical

1714

Issue of the periodical within the volume

Jan

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

12

Pages from-to

464548

UT code for WoS article

001134754400001

EID of the result in the Scopus database

2-s2.0-85178139487