Assessment of pDCs functional capacity upon exposure to tumor-derived soluble factors
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064203%3A_____%2F24%3A10483764" target="_blank" >RIV/00064203:_____/24:10483764 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11110/24:10483764
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=kZ8ydrOR15" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=kZ8ydrOR15</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/bs.mcb.2024.07.002" target="_blank" >10.1016/bs.mcb.2024.07.002</a>
Alternative languages
Result language
angličtina
Original language name
Assessment of pDCs functional capacity upon exposure to tumor-derived soluble factors
Original language description
Plasmacytoid dendritic cells (pDCs) are a minority subset of dendritic cells that despite their tiny quantity play an important role in the immune system, especially in antiviral immunity. They are known mostly as the major producers of type I IFN, which they secrete upon stimulation of endosomal Toll-like receptors 7 and 9 with viral RNA and DNA. However, the functionality of pDCs is more complex, as they were shown to be also involved in autoimmunity, inflammation, and cancer. In the context of the tumor microenvironment, pDCs mostly show substantial functional defects and thus contribute to establishing immunosuppressive micromilieu. Indeed, tumor-infiltrating pDCs were shown to be predominantly pro-tumorigenic, with reduced ability to produce IFNα and capacity to prime regulatory T cells via the ICOS/ICOS-L pathway. Here we describe in detail a method to assess the functional capacity of pDCs upon exposure to tumor-derived cell culture supernatants. The same technique can be implemented with minimal variations to test any soluble factor's impact on pDC phenotype and function.
Czech name
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Czech description
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Classification
Type
J<sub>SC</sub> - Article in a specialist periodical, which is included in the SCOPUS database
CEP classification
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OECD FORD branch
30102 - Immunology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Methods in Cell Biology
ISSN
0091-679X
e-ISSN
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Volume of the periodical
2024
Issue of the periodical within the volume
189
Country of publishing house
US - UNITED STATES
Number of pages
12
Pages from-to
85-96
UT code for WoS article
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EID of the result in the Scopus database
2-s2.0-85201890179