Plasmacytoid Dendritic Cells in Patients with MGUS and Multiple Myeloma

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17110%2F21%3AA2202CS8" target="_blank" >RIV/61988987:17110/21:A2202CS8 - isvavai.cz</a>

Alternative codes found

RIV/00843989:_____/21:E0109190 RIV/65269705:_____/21:00075845 RIV/00216224:14110/21:00120139

Result on the web

<a href="https://www.webofscience.com/wos/woscc/full-record/WOS:000689192700001" target="_blank" >https://www.webofscience.com/wos/woscc/full-record/WOS:000689192700001</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/jcm10163717" target="_blank" >10.3390/jcm10163717</a>

Result language

angličtina

Original language name

Plasmacytoid Dendritic Cells in Patients with MGUS and Multiple Myeloma

Original language description

Background: Plasmacytoid dendritic cells (pDCs) play prominent roles in mediating innate and adaptive immune responses. However, it is unclear how pDCs contribute to the immunosuppressive tumor microenvironment described in multiple myeloma (MM). Methods: Newly diagnosed myeloma patients (MM, n = 37) were analyzed to determine the pDC counts in comparison to peripheral blood (PB, n = 53) and bone marrow (BM, n = 10) samples of age-matched healthy donors (HD) using flow cytometry. Second, proliferation of myeloma tumor cells in the presence of freshly isolated pDCs was examined. Third, production of IFN alpha by pDCs co-cultured with MM cells was determined by intracellular staining. Results: We found a highly significant reduction of circulating pDCs (p < 0.0001) and in bone marrow (p < 0.0001) of MM patients compared to HD. We also observed a significant decrease of pDCs (p = 0.004) in BM in patients with monoclonal gammopathy of undetermined significance (MGUS, n = 12). Importantly, we determined that pDCs promote proliferation specifically of MM cells and not the stromal cells and that pDCs secrete IFN alpha upon co-culture with MM tumor cells. Conclusions: Our results show altered pDC frequencies in the BM microenvironment in MGUS and MM patients at diagnosis. We showed the tumor-promoting function of pDCs that may mediate immune deficiencies affecting long-term disease control and treatment outcome.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30218 - General and internal medicine

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Clinical Medicine

ISSN

2077-0383

e-ISSN

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Volume of the periodical

10

Issue of the periodical within the volume

16

Country of publishing house

CH - SWITZERLAND

Number of pages

11

Pages from-to

1-11

UT code for WoS article

000689192700001

EID of the result in the Scopus database

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