Minoxidil decreases collagen I deposition and tissue-like contraction in clubfoot-derived cells: a way to improve conservative treatment of relapsed clubfoot?

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064211%3A_____%2F20%3AW0000010" target="_blank" >RIV/00064211:_____/20:W0000010 - isvavai.cz</a>

Alternative codes found

RIV/67985823:_____/21:00547576 RIV/00216208:11510/21:10415820 RIV/00216208:11110/21:10415820 RIV/00064211:_____/21:W0000052 RIV/00216208:11130/21:10415820

Result on the web

<a href="https://oadoi.org/10.1080/03008207.2020.1816992" target="_blank" >https://oadoi.org/10.1080/03008207.2020.1816992</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/03008207.2020.1816992" target="_blank" >10.1080/03008207.2020.1816992</a>

Result language

angličtina

Original language name

Minoxidil decreases collagen I deposition and tissue-like contraction in clubfoot-derived cells: a way to improve conservative treatment of relapsed clubfoot?

Original language description

Aim Clubfoot is a congenital deformity affecting the musculoskeletal system, resulting in contracted and stiff tissue in the medial part of the foot. Minoxidil (MXD) has an inhibitory effect on lysyl hydroxylase, which influences the quality of extracellular matrix crosslinking, and could therefore be used to reduce the stiffness and to improve the flexibility of the tissue. We assessed thein vitroantifibrotic effects of minoxidil on clubfoot-derived cells. Methods Cell viability and proliferation were quantified by xCELLigence, MTS, and LIVE/DEAD assays. The amount of collagen I deposited into the extracellular matrix was quantified using immunofluorescence with subsequent image segmentation analysis, hydroxyproline assay, and Second Harmonic Generation imaging. Extracellular matrix contraction was studied in a 3D model of cell-populated collagen gel lattices. Results MXD concentrations of 0.25, 0.5, and 0.75 mM inhibited the cell proliferation in a concentration-dependent manner without causing a cytotoxic effect. Exposure to >= 0.5 mM MXD resulted in a decrease in collagen type I accumulation after 8 and 21 days in culture. Changes in collagen fiber assembly were observed by immunofluorescence microscopy and nonlinear optical microscopy (second harmonic generation). MXD also inhibited the contraction of cell-populated collagen lattices (0.5 mM by 22%; 0.75 mM by 28%). Conclusions Minoxidil exerts anin vitroinhibitory effect on the cell proliferation, collagen accumulation, and extracellular matrix contraction processes that are associated with clubfoot fibrosis. This study provides important preliminary results demonstrating the potential relevance of MXD for adjuvant pharmacological therapy in standard treatment of relapsed clubfoot.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30211 - Orthopaedics

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

CONNECTIVE TISSUE RESEARCH

ISSN

0300-8207

e-ISSN

1607-8438

Volume of the periodical

—

Issue of the periodical within the volume

20 Sep 2020

Country of publishing house

US - UNITED STATES

Number of pages

16

Pages from-to

-

UT code for WoS article

000571321300001

EID of the result in the Scopus database

2-s2.0-85091144762