3D printed stratospheric probe as a platform for determination of DNA damage based on carbon quantum dots/DNA complex fluorescence increase

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00098639%3A_____%2F16%3AN0000010" target="_blank" >RIV/00098639:_____/16:N0000010 - isvavai.cz</a>

Alternative codes found

RIV/62156489:43210/16:43909553 RIV/00216305:26620/16:PU120121

Result on the web

<a href="https://link.springer.com/article/10.1007%2Fs00706-016-1705-y" target="_blank" >https://link.springer.com/article/10.1007%2Fs00706-016-1705-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00706-016-1705-y" target="_blank" >10.1007/s00706-016-1705-y</a>

Result language

angličtina

Original language name

3D printed stratospheric probe as a platform for determination of DNA damage based on carbon quantum dots/DNA complex fluorescence increase

Original language description

We present a utilization of carbon quantum dots (CQDs), passivated with polyethylene glycol as a fluorescent recognition probe for DNA damage. Synthesized CQDs were characterized in detail, using optical and electrochemical methods. Further, fluorescent behavior of CQDs was monitored in the presence of genomic DNA, isolated from Staphylococcus aureus. In laboratory conditions, after 30 min of exposure to UV irradiation (lambda = 254 nm), the DNA/CQDs complex significantly increased its fluorescence. Further, stratospheric probe was suggested and crafted by using technology of 3D printing (acrylonitrile-butadiene-styrene as a material). CQDs were exploited to evaluate the DNA damage in stratospheric conditions (up to 20,000 m) by determination of fluorescence increase (lambda (exc) = 245 nm, lambda (em) = 400 nm), together with other parameters (temperature, humidity, altitude, pressure, UV intensity, and X-ray irradiation). The obtained data showed that the sensor utilizing the DNA/CQDs was able to identify the DNA damage, together with external conditions. It was shown the proposed concept is able to operate at temperatures lower than -70 A degrees C. The proposed protocol may by applicable as a biosensor for long-term space missions, like international space station, missions to the Moon or Mars.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10406 - Analytical chemistry

Project

—

Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Monatshefte für Chemie - Chemical Monthly

ISSN

0026-9247

e-ISSN

1434-4475

Volume of the periodical

147

Issue of the periodical within the volume

5

Country of publishing house

AT - AUSTRIA

Number of pages

8

Pages from-to

873-880

UT code for WoS article

000374172200005

EID of the result in the Scopus database

—