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3D printed stratospheric probe as a platform for determination of DNA damage based on carbon quantum dots/DNA complex fluorescence increase

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F16%3A43909553" target="_blank" >RIV/62156489:43210/16:43909553 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216305:26620/16:PU120121 RIV/00098639:_____/16:N0000010

  • Result on the web

    <a href="http://dx.doi.org/10.1007/s00706-016-1705-y" target="_blank" >http://dx.doi.org/10.1007/s00706-016-1705-y</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/s00706-016-1705-y" target="_blank" >10.1007/s00706-016-1705-y</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    3D printed stratospheric probe as a platform for determination of DNA damage based on carbon quantum dots/DNA complex fluorescence increase

  • Original language description

    We present a utilization of carbon quantum dots (CQDs), passivated with polyethylene glycol as a fluorescent recognition probe for DNA damage. Synthesized CQDs were characterized in detail, using optical and electrochemical methods. Further, fluorescent behavior of CQDs was monitored in the presence of genomic DNA, isolated from Staphylococcus aureus. In laboratory conditions, after 30 min of exposure to UV irradiation (lambda = 254 nm), the DNA/CQDs complex significantly increased its fluorescence. Further, stratospheric probe was suggested and crafted by using technology of 3D printing (acrylonitrile-butadiene-styrene as a material). CQDs were exploited to evaluate the DNA damage in stratospheric conditions (up to 20,000 m) by determination of fluorescence increase (lambda (exc) = 245 nm, lambda (em) = 400 nm), together with other parameters (temperature, humidity, altitude, pressure, UV intensity, and X-ray irradiation). The obtained data showed that the sensor utilizing the DNA/CQDs was able to identify the DNA damage, together with external conditions. It was shown the proposed concept is able to operate at temperatures lower than -70 A degrees C. The proposed protocol may by applicable as a biosensor for long-term space missions, like international space station, missions to the Moon or Mars.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CB - Analytical chemistry, separation

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/LO1401" target="_blank" >LO1401: Interdisciplinary Research of Wireless Technologies</a><br>

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2016

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Monatshefte für Chemie - Chemical Monthly

  • ISSN

    0026-9247

  • e-ISSN

  • Volume of the periodical

    147

  • Issue of the periodical within the volume

    5

  • Country of publishing house

    AT - AUSTRIA

  • Number of pages

    8

  • Pages from-to

    873-880

  • UT code for WoS article

    000374172200005

  • EID of the result in the Scopus database

    2-s2.0-84960350670