Analysis of Vancomycin-Resistant Enterococci in Hemato-Oncological Patients
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00098892%3A_____%2F20%3AN0000057" target="_blank" >RIV/00098892:_____/20:N0000057 - isvavai.cz</a>
Result on the web
<a href="https://www.mdpi.com/2079-6382/9/11/785/htm" target="_blank" >https://www.mdpi.com/2079-6382/9/11/785/htm</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/antibiotics9110785" target="_blank" >10.3390/antibiotics9110785</a>
Alternative languages
Result language
angličtina
Original language name
Analysis of Vancomycin-Resistant Enterococci in Hemato-Oncological Patients
Original language description
Enterococci are important bacterial pathogens, and their significance is even greater in the case of vancomycin-resistant enterococci (VRE). The study analyzed the presence of VRE in the gastrointestinal tract (GIT) of hemato-oncological patients. Active screening using selective agars yielded VRE for phenotypic and genotypic analyses. Isolated strains were identified with MALDI-TOF MS, (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry) their susceptibility to antibiotics was tested, and resistance genes (vanA, vanB, vanC-1, vanC2-C3) and genes encoding virulence factors (asa1, gelE, cylA, esp, hyl) were detected. Pulsed-field gel electrophoresis was used to assess the relationship of the isolated strains. Over a period of three years, 103 VanA-type VRE were identified in 1405 hemato-oncological patients. The most frequently detected virulence factor was extracellular surface protein (84%), followed by hyaluronidase (40%). Unique restriction profiles were observed in 33% of strains; clonality was detected in 67% of isolates. The study found that 7% of hemato-oncological patients carried VRE in their GIT. In all cases, the species identified was Enterococcus faecium. No clone persisted for the entire 3-year study period. However, genetically different clusters were observed for shorter periods of time, no longer than eight months, with identical VRE spreading among patients.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
<a href="/en/project/NV18-05-00340" target="_blank" >NV18-05-00340: Epidemiology of vancomycin-resistant enterococci in the Czech Republic: One Health concept</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Antibiotics-Basel
ISSN
2079-6382
e-ISSN
2079-6382
Volume of the periodical
9
Issue of the periodical within the volume
11
Country of publishing house
CH - SWITZERLAND
Number of pages
10
Pages from-to
785
UT code for WoS article
000592778100001
EID of the result in the Scopus database
2-s2.0-85096055853