Analysis of Vancomycin-Restistant Enterococci in Hemato-Oncological Patiens

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F20%3A73605696" target="_blank" >RIV/61989592:15110/20:73605696 - isvavai.cz</a>

Result on the web

<a href="https://www.mdpi.com/2079-6382/9/11/785" target="_blank" >https://www.mdpi.com/2079-6382/9/11/785</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/antibiotics9110785" target="_blank" >10.3390/antibiotics9110785</a>

Result language

angličtina

Original language name

Analysis of Vancomycin-Restistant Enterococci in Hemato-Oncological Patiens

Original language description

Enterococci are important bacterial pathogens, and their significance is even greater in the case of vancomycin-resistant enterococci (VRE). The study analyzed the presence of VRE in the gastrointestinal tract (GIT) of hemato-oncological patients. Active screening using selective agars yielded VRE for phenotypic and genotypic analyses. Isolated strains were identified with MALDI-TOF MS, (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry) their susceptibility to antibiotics was tested, and resistance genes (vanA, vanB, vanC-1, vanC2-C3) and genes encoding virulence factors (asa1, gelE, cylA, esp, hyl) were detected. Pulsed-field gel electrophoresis was used to assess the relationship of the isolated strains. Over a period of three years, 103 VanA-type VRE were identified in 1405 hemato-oncological patients. The most frequently detected virulence factor was extracellular surface protein (84%), followed by hyaluronidase (40%). Unique restriction profiles were observed in 33% of strains; clonality was detected in 67% of isolates. The study found that 7% of hemato-oncological patients carried VRE in their GIT. In all cases, the species identified was Enterococcus faecium. No clone persisted for the entire 3-year study period. However, genetically different clusters were observed for shorter periods of time, no longer than eight months, with identical VRE spreading among patients

Czech name

—

Czech description

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Type

J_SC - Article in a specialist periodical, which is included in the SCOPUS database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/NV18-05-00340" target="_blank" >NV18-05-00340: Epidemiology of vancomycin-resistant enterococci in the Czech Republic: One Health concept</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Antibiotics-Basel

ISSN

2079-6382

e-ISSN

—

Volume of the periodical

9

Issue of the periodical within the volume

11

Country of publishing house

CH - SWITZERLAND

Number of pages

10

Pages from-to

1-10

UT code for WoS article

000592778100001

EID of the result in the Scopus database

2-s2.0-85096055853