Multiplex Immunofluorescent Analysis of Alpha-Synuclein in Nigral Lewy Bodies With Heat-Induced Antibody Stripping Reveals an Intricate Multilayered Structure
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00098892%3A_____%2F25%3A10159275" target="_blank" >RIV/00098892:_____/25:10159275 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15110/25:73632723
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/10.1111/nan.70024" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1111/nan.70024</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/nan.70024" target="_blank" >10.1111/nan.70024</a>
Alternative languages
Result language
angličtina
Original language name
Multiplex Immunofluorescent Analysis of Alpha-Synuclein in Nigral Lewy Bodies With Heat-Induced Antibody Stripping Reveals an Intricate Multilayered Structure
Original language description
Lewy bodies, the hallmark intracellular inclusions in Parkinson’s disease and dementia with Lewy bodies, exhibit complex architecture comprising multiple alpha-synuclein proteoforms, ubiquitin, cytoskeletal elements, and organelles. In this study, we applied a cost-effective and tissue-conserving multiplex immunofluorescence technique using heat-induced antibody stripping to examine the structural organization of nigral Lewy bodies in postmortem brain tissue from 11 patients with Lewy body disease. Using a panel of well-characterized alpha-synuclein antibodies targeting different domains, we analysed 71 brainstem Lewy bodies and identified a consistent concentric “onion-like” architecture. The C-terminal and N-terminal domains were predominantly localized to the outer shell, while the NAC domain displayed diffuse distribution. Notably, different alpha-synuclein epitopes showed variable colocalization, reflecting the influence of truncation and post-translational modifications on antibody binding. In contrast, pale bodies lacked clear structural organization. Our findings highlight the structural heterogeneity of Lewy bodies and support the use of multiple domain-specific antibodies to reliably identify and study these inclusions. This multiplex approach offers a scalable and accessible method for detailed spatial proteoform mapping, with potential applications in both research and neuropathological diagnostics.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30109 - Pathology
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Neuropathology and Applied Neurobiology
ISSN
0305-1846
e-ISSN
1365-2990
Volume of the periodical
51
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
5
Pages from-to
"e70024"
UT code for WoS article
001498975800001
EID of the result in the Scopus database
2-s2.0-105006853751