Determination of adenosine deaminase activity in human erythocytes by on-column Capillary isotachophoresis - capillary zone electrophoresis in the present of electrosmotic flow

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00098892%3A_____%2F99%3A00000003" target="_blank" >RIV/00098892:_____/99:00000003 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Determination of adenosine deaminase activity in human erythocytes by on-column Capillary isotachophoresis - capillary zone electrophoresis in the present of electrosmotic flow

Original language description

Transient capillary isotachophoresis - capillary electrophoresis at the presenseof electroosmotic flow was utilized for the measurement of adenosine deaminase activity in human erythocytes. Phosphates, a dominant anios of the sample matrice were used asleading ions for transint isotachophoresis and borates (0.3 M, pH 10) were used as terminating ions and background electrolyte for capillary zone electrophoresis. Final experimental conditions allowed to inject 70% of the total capillary volume (1.45 mL)with the sample. Product of enzymatic conversions present in the sample in low-micromoral range were determined by the use of optimized conditions. Limit of detection was 28 nM using UV detection at 202 nm. Presented data showed that CITP-CZE can be performed in uncoated capillaries at the presence of strong EOF.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FG - Paediatrics

OECD FORD branch

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Project

<a href="/en/project/IZ3439" target="_blank" >IZ3439: Diagnostics of inherited disorders of purine and pyrimidine metabolism usíng capillary electrophoresis.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

1999

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Electrophoresis

ISSN

0173-0835

e-ISSN

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Volume of the periodical

20

Issue of the periodical within the volume

3

Country of publishing house

DE - GERMANY

Number of pages

5

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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