Determination of adenosine deaminase activity in human erythrocytes by on-column capillary isotachophoresis capillary zone electrophoresis in the presence of electroosmotic flow

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F99%3A00000732" target="_blank" >RIV/61989592:15310/99:00000732 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15110/99:00000246

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Determination of adenosine deaminase activity in human erythrocytes by on-column capillary isotachophoresis capillary zone electrophoresis in the presence of electroosmotic flow

Original language description

Transient capillary isotachophoresis (CITP)-capillary zone electrophoresis (CZE) in presence of electroosmotic flow (EOF) was utilized for the measurement of adenosine deaminase activity in human erythrocytes. Phosphates, dominant anions of the sample matrix, were used as leading ions for transient isotachophoresis, and berates (0.3 M, pH 10) were used as terminating ions and background electrolyte for CZE. Final experimental conditions made it possible to inject 70% of the total capillary volume (1.45mu L) with the sample. Enzymatic conversion products (inosine and hypoxanthine), present in the sample in the low-micromolar range, were determined using optimized conditions. The limit of detection was 28 nm using UV detection at 202 nm. The presented data shows that CITP-CZE can be performed in uncoated capillaries in the presence of strong EOF.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

1999

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

ELECTROPHORESIS

ISSN

0173-0835

e-ISSN

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Volume of the periodical

20

Issue of the periodical within the volume

3

Country of publishing house

XX - stateless person

Number of pages

5

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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