Properties of neural crest-like cells differentiated from human embryonic stem cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F14%3A00061220" target="_blank" >RIV/00159816:_____/14:00061220 - isvavai.cz</a>

Alternative codes found

RIV/68081707:_____/14:00435410 RIV/68081707:_____/14:00436649 RIV/67985904:_____/14:00436649 RIV/00216224:14110/14:00074170 RIV/67985904:_____/14:00435410

Result on the web

<a href="http://fb.cuni.cz/file/5742/fb2014a0029.pdf" target="_blank" >http://fb.cuni.cz/file/5742/fb2014a0029.pdf</a>

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Properties of neural crest-like cells differentiated from human embryonic stem cells

Original language description

Neural crest cells (NCCs) derive early in vertebrate ontogenesis from neural tube as a population of migratory cells with exquisite differentiation potential. Abnormalities in NCC behaviour are cause of debilitating diseases including cancers and a spectrum of neurocristopathies. Thanks to their multilineage differentiation capacity NCCs offer a cell source for regenerative medicine. Both these aspects make NCC biology an important issue to study, which can currently be addressed using methodologies based on pluripotent stem cells. Here we contributed to understanding the biology of human NCCs by refining the protocol for differentiation/propagation of NCClike cells from human embryonic stem cells and by characterizing the molecular and functional phenotype of such cells. Most importantly, we improved formulation of media for NCC culture, we found that poly-L-ornithine combined with fibronectin provide good support for NCC growth, we unravelled the tendency of cultured NCCs to maintain

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

—

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Folia biologica (Praha)

ISSN

0015-5500

e-ISSN

—

Volume of the periodical

60

Issue of the periodical within the volume

S1

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

9

Pages from-to

30 - 38

UT code for WoS article

000343275800005

EID of the result in the Scopus database

—